细胞融合高效菌株对氧乐果生产废水中有机磷的去除  

REMOVAL OF OMETHOATE-PRODUCING WASTEWATER'S ORGANOPHOSPHORUS WITH EFFICIENT CELL FUSION STRAIN

作　　者：庄桂[1] 刘云焕[1] 贺以乐 

机构地区：[1]河南工业大学生物工程学院,河南郑州450052 [2]沙隆达郑州农药有限公司,河南郑州450009

出　　处：《环境工程》2008年第S1期13-15,20,共4页Environmental Engineering

基　　金：河南省科技厅科技攻关项目(0624440036)

摘　　要：两株具有单重抗药性的突变菌株S-8菌株(Str+,Kan-)和K-17菌株(Str-,Kan+),其活性污泥对氧乐果生产废水有机磷的去除率分别达58%和51%,对COD的去除率分别达78%和82%。以S-8菌株和K-17菌株作为亲本菌株进行原生质体融合,其正交试验的优化条件为:PEG浓度40%;促融时间4min;用0.015 mol/LCa2+处理;pH 10.0;添加20μL/ml细胞壁再生引物,融合率达4.617×10-5。用双层药物平板筛选融合细胞,获取了既能降解有机磷又能降低COD值的融合子F2。结果表明,用融合子F2菌株增殖而成的活性污泥对上述氧乐果生产废水有机磷的去除率达78%,COD的去除率达88%。Two single resistant mutants,i.e.S-8 strain(Str^+,Kan^-) and K-17 strain(Str^-,Kan^+) in active sludges could remove 58% and 51% organophosphorus,78% and 82% COD of the omethoate-producing wasterwater respectively.Through orthorhombic test of protoplast fusion technique using the two mutants as parent strains,the optimal conditions of protoplast fusion under which the rate of fusion was up to 4.617×10^(-5) were confirmed as follows: 40%;time of fusion 4min;[Ca^(2+)] 0.015mol/L;pH 10.0;cell wall regeneration inducer 20μl/ mL.By the means of double layer drug plate method to screen the fusion cells,the fusion strain F_2 was gained,which could degrade organophosphorus and reduce COD at the same time.The results showed that the active sludge multiplying from the fusion strain F_2 could remove 78% organophosphorus,and 88% COD of aforementioned omethoate-producing wasterwater respectively.

关 键 词：细胞融合 有机磷 氧乐果降解 活性污泥 废水处理 

分 类 号：X703[环境科学与工程—环境工程]