绵羊HOXC8基因的DNA甲基化研究  

STUDY ON DNA METHYLATION OF SHEEP HOXC8

作　　者：赵静[1] 张立岭[1,2] 陈琦[3] 巴图 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018 [2]海南大学动物科学系,海口570228 [3]内蒙古医学院,呼和浩特010110 [4]内蒙古农牧科学院畜牧研究所,呼和浩特010030

出　　处：《内蒙古农业大学学报（自然科学版）》2011年第2期15-20,共6页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(30960245);国家十一五科技支撑项目(2006BDA13B08)

摘　　要：本研究测定了蒙古羊和德国美利奴肉羊,道塞特肉羊,澳洲美利奴细毛羊Hoxc8全基因序列。蒙古羊与这3个外国品种的Hoxc8基因,只有内含子存在8个碱基差异。但是exon-1的甲基化模式存在极大差异。蒙古羊14枚胸椎个体的甲基化程度高于13枚胸椎个体。蒙古羊甲基化程度普遍高于外国品种。蒙古羊特有的可遗传甲基化位点共有6个(5,69,1,62,6,27),国外品种在这些位置不发生甲基化。并且,这6个位点的甲基化程度高低可能决定了第14枚胸椎的长短和骨化程度。Hoxc8 gene of Mongolian,German Merino,Dorset and Australian Merino were sequenced.Among these breeds,there were eight differences of the bases in the intron but much more varies on the methylation patterns in the exon-1 in the genes.The level of the methylation in 14 vertebrae individuals was higher than that in 13 vertebrae individuals for Mongolian sheep.Generally,the methylation level in the Mongolian sheep was higher than that in the other 3 foreign breeds.There were 6 methylation locations in Mongolian sheep only,but without any methylation in the same locations among the foreign breeds.The results indicated that the methylation level higher or lower for the 6 methylation locations could determined the length and bone strength.

关 键 词：绵羊 Hoxc8 CPG 亚硫酸氢盐测序PCR 

分 类 号：S826.1[农业科学—畜牧学]