Effects of H_2O_2 on Oxygen Supply in the Process of DCA Fermentation  

作　　者：黄英明 焦鹏 李书良 华玉涛 曹竹安 

出　　处：《Tsinghua Science and Technology》2002年第5期442-447,共6页清华大学学报（自然科学版（英文版）

基　　金：Supported by the National Natural Science Foundationof China (Nos.3 0 0 0 0 0 0 3 and 2 99760 2 2 )

摘　　要：Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by adding hydrogen peroxide (H_2O_2) to the fermentation system. This paper shows that H_2O_2 can not only enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of α, ω-oxidation. When C. tropicalis was cultivated in a 3-liter bioreactor using the combination of aeration and hydrogen peroxide feeding, the DCA yield increased about 10% except at the beginning of H_2O_2 feeding. The experiments showed that the maximum activities of P450 could be induced at 2 mmol·L -1 H_2O_2. By adding H_2O_2, the DCA yield in a 22-liter bioreactor was increased 25.3% to 153.9 g/L.Long chain dicarboxylic acid (DCA) produced by Candida tropicalis is produced in an aerobic viscous fermentation system. The gas-liquid transport resistance can be overcome and the oxygen supply can be increased by adding hydrogen peroxide (H_2O_2) to the fermentation system. This paper shows that H_2O_2 can not only enhance the oxygen supply but also change the metabolism by inducing cytochrome P450, the key enzyme of α, ω-oxidation. When C. tropicalis was cultivated in a 3-liter bioreactor using the combination of aeration and hydrogen peroxide feeding, the DCA yield increased about 10% except at the beginning of H_2O_2 feeding. The experiments showed that the maximum activities of P450 could be induced at 2 mmol·L -1 H_2O_2. By adding H_2O_2, the DCA yield in a 22-liter bioreactor was increased 25.3% to 153.9 g/L.

关 键 词：dicarboxylic acid C. tropicalis oxygen supply hydrogen peroxide cytochrome P450 

分 类 号：TQ920[轻工技术与工程—发酵工程]