Menadione-induced apoptosis and its mechanism in plants  

作　　者：SUN Yingli ZHOU Jun DAI Yaoren ZHAI Zhonghe 

机构地区：[1]College of Life Sciences,Peking University,Beijing 100871,China [2]Biology Department,Tsinghua University,Beijing 100084,China

出　　处：《Chinese Science Bulletin》2000年第4期350-354,共5页

摘　　要：Menadione can induce apoptosis in tobacco protoplasts, Typical characteristics are detected including the condensation of chromatin, the formation of apoptotic bodies and the degradation of genomic DNA into 'DNA ladder'. Specific DNase is activated during this process. Ca2+ and Mg2+ are necessary for its activation, while 2n2+ and EDTA (Ethylenediaminetetraacetic acid) can inhibit its activation. The fragmentation of DNA and lamin can be inhibited by DEVD (Ac-Asp -Glu- Val- Asp-aldehyde). The fragmentation of lamin can also be inhibited by PMSF (Phenylmethylsulfonyl fluoride) and CH (Cyclohexinide). These results show that activation of specific DNase and proteases is involved in menadion-induced apoptosis in plants.Menadione can induce apoptosis in tobacco protoplasts. Typical characteristics are detected including the condensation of chromatin, the formation of apoptotic bodies and the degradation of genomic DNA into “DNA ladder”. Specific DNase is activated during this process. Ca2+ and Mg2+ are necessary for its activation, while Zn2+ and EDTA (Ethylenediaminetetraacetic acid) can inhibit its activation. The fragmentation of DNA and lamin can be inhibited by DEVD (Ac-Asp -Glu- Val- Asp-aldehyde). The fragmentation of lamin can also be inhibited by PMSF (Phenylmethylsulfonyl fluoride) and CH (Cyclohexinide). These results show that activation of specific DNase and proteases is involved in menadion-induced apoptosis in plants.

关 键 词：TOBACCO PROTOPLASTS MENADIONE apoptosis DNA FRAGMENTATION LAMIN protease. 

分 类 号：Q943[生物学—植物学]