In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence  

作　　者：陈春英 章佩群 柴之芳 李光城 黄宇营 

机构地区：[1]Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China [2]Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100080, China

出　　处：《Science China Mathematics》2000年第1期88-92,共5页中国科学：数学（英文版）

摘　　要：A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique forin situ analysis of trace elements in protein bands.A method using synchrotron radiation X-ray fluorescence analysis to in situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed a-long polyacrylamide gel. The results showed that the protein fraction of peak III in cvtosol was mainly composed of metal ion Zn-associated proteins, being in agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique for in situ analysis of trace elements in protein bands.

关 键 词：SYNCHROTRON radiation X-ray fluorescence ANALYSIS in SITU ANALYSIS METALLOPROTEIN electrophoretic separation. 

分 类 号：O434.1[机械工程—光学工程]