Effect of Lanthanum Citrate on Two Cell Lines: Human Lung Cancer Cells PG and Human Gastric Carcinoma Cells BGC-823  

作　　者：LI Xiao bin 1, ZHOU Ai ru 1, YU Wen hua 1, CHEN Xing an 2( 1. Department of Biochemistry & Molecular Biology, Beijing Medical University, Beijing 100083, China 2. National Center of Hygienic Evaluation for Rare Earth Element Development and Utilization, Beijing 100088, China ) 

出　　处：《Journal of Rare Earths》2000年第2期139-139,共1页稀土学报（英文版）

摘　　要：The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.

关 键 词：rare earths LaCit PG cells BGC  823 cells growth inhibition 

分 类 号：R735.2[医药卫生—肿瘤]