炎性刺激下张应力对成骨细胞核心结合因子α1表达的影响  

Effects of Tensile Strain on the Expression of Runx2/Cbfα1 in Osteoblasts Cultivated with Inflammatory Stimulus

作　　者：胡荣党[1] 叶洁[1] 徐春燕[1] 

机构地区：[1]温州医学院附属口腔医院正畸科,温州325027

出　　处：《四川大学学报（医学版）》2011年第6期827-830,共4页Journal of Sichuan University(Medical Sciences)

基　　金：浙江省自然科学基金(Y207360)资助

摘　　要：目的观察成骨细胞在牙龈卟啉菌脂多糖(Pg-LPS)刺激单核巨噬细胞上清培养下张应力对其核心结合因子α1(Runx2/Cbfα1)表达的影响。方法成骨细胞MC3T3-E1于Pg-LPS刺激单核巨噬细胞上清培养24h后,应用四点弯曲加力装置对细胞分别加载1000μstrain和3000μstrain循环单轴张应力0h、0.5h、1h、2h、3h、6h,采用实时荧光PCR检测细胞Runx2/Cbfα1mRNA的表达差异性。同时设非炎性刺激组为对照组。结果机械牵张力刺激下,炎性刺激组细胞和非炎性刺激组细胞Runx2/Cbfα1mRNA表达趋势相似,0.5h后升高,6h最高;炎性刺激组细胞各时点Runx2/Cbfα1mRNA表达低于非炎性刺激组(P<0.05);不同应力作用下,前3h细胞Runx2/Cbfα1mRNA表达未见明显差异,6h时3000μstrain组细胞表达高于1000μstrain组(P<0.05)。结论炎症刺激可抑制应力下成骨细胞Runx2/Cbfα1的表达,提示临床上对牙周炎静止期患者施力需谨慎。Objective To determine the effects of tensile strain on the expression of core binding factor α1（Runx2/Cbfα1） in osteoblasts cultivated in Pg-LPS stimulated RAW264.7 culture supernatant.Methods Osteoblasts MC3T3-E1 were cultivated in LPS stimulated monocyte culture supernatant for 24 h.Then,a four-point bending device was used to perform a single period of tensile strain（1000 μ strain,3000 μ strain respectively） on osteoblasts for 0 h,0.5 h,1 h,2 h,3 h,6 h.Non-inflammatory stimuli were set up as control.The expression of Runx2/Cbfα1 mRNA in MC3T3-E1 cells was examined by real-time RT-PCR.Results With the mechanical loading,the expression of Runx2/Cbfα1 mRNA increased in both inflammatory and non-inflammatory groups of osteoblasts and reached the peak after 6 h.The inflammatory group had lower expression of Runx2/Cbfα1 mRNA than that of the non-inflammatory group（P0.05）.The varied strain level did not change the expression of Runx2/Cbfα1 mRNA within 3 hours.But after 6 hours,the 3000 μ strain induced higher expression of Runx2/Cbfα1 mRNA than the 1000 μ strain.Conclusion Inflammation inhibits the tensile strain induced expression of Runx2/Cbfα1 in osteoblasts.Precaution needs to be paid to orthodontic treatment on patients with quiescent periodontitis.

关 键 词：脂多糖 单核细胞 机械牵张 成骨细胞 核心结合因子Α1 

分 类 号：R363[医药卫生—病理学]