青春期大鼠升高咬合后髁突软骨细胞中TGF-β1的表达变化  

Effect of Occlusion Vertical Rising on the Expression of TGF-β1 in Condylar Cartilage of Adolescent Rats

作　　者：周婷[1] 夏悦[1] 杨正[1] 罗金华[2] 杨四维[1] 

机构地区：[1]泸州医学院附属口腔医院正畸科,泸州646000 [2]巴中市中心医院内科,巴中636600

出　　处：《四川大学学报（医学版）》2011年第6期842-845,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨青春期大鼠咬合升高后髁突软骨中转化生长因子β1(TGF-β1)表达的变化。方法选取40只5周龄Wistar雄性大鼠,随机分为实验组和对照组,每组20只。实验组双侧上颌磨牙用树脂板升高咬合。分别于术后7、14、21及28d,取其左侧髁突,用免疫组织化学SABC法检测大鼠髁突软骨中TGF-β1的表达变化。结果与对照组相比,实验组TGF-β1在7d时表达最少,14d和21d逐渐增加,到28d时增加到最高(P<0.05)。结论咬合升高后导致髁突软骨中TGF-β1表达增强,TGF-β1可能参与了大鼠髁突软骨的适应性改建。Objective To determine the expression and localization of transforming growth factor（TGF）-β1 in condylar cartilage of adolescent rats following occlusion vertical rising procedures.Methods Forty male rats of 5 weeks of age were randomly divided into the experiment group（n=20） and the control group（n=20）.The rats in the experiment group were fitted with upper posterior bite rising splints on their upper molar.Five rats from each group were killed at 7,14,21 and 28 days after the procedures,and their left condylar cartilages were taken for the determination of the expression of TGF-β1 through SABC.Results The rats in the experiment group had significantly less expression of TGF-β1 in condylar cartilage than the controls.The rats in the experiment group had the lowest TGF-β1 7 day after the procedure.The level of TGF-β1 increased over time.More TGF-β1 positive cells were found in the rats of the experiment group than those in the control 28 days after the procedure.Conclusion Occlusal vertical rising stimulates the expression of TGF-β1 in condylar cartilage,which may be part of the adaptive remodeling of condylar cartilage in adolescent rats.

关 键 词：髁突软骨 板 骨改建 转化生长因子-Β1 

分 类 号：R783.5[医药卫生—口腔医学]