Indirect neurotrophic effect of neuropeptide ZNC (C) PR on PC12 cells via peptide-stimulation of C6 cells-  

神经肽ZNC(C)PR通过刺激C6细胞而对PC12起间接营养作用(英文)

作　　者：阎庆武[1] 甄晓光[1] 王桐喜 徐侃彦 董明[1] 杜雨苍[1] 

机构地区：[1]中国科学院上海生物化学研究所

出　　处：《Acta Pharmacologica Sinica》2000年第5期28-32,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (№ 39570157);the State Key Program (№ P8514);Shanghai Research Center of Life Science (№ 95 JC 14007).

摘　　要：AIM: To understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR and its effect on which could affect both growth and apoptosis of C6 cells. METHODS: Effects of ZNC (C) PR-treated C6 conditioned medium was observed on on culture of PC12 cells. The development of PC12 cells was determined by ratio of neurite-bearing cells in the total cells. The specific binding of ZNC (C) PR on C6 cells was determined by ra-dioligand binding assay (RBA). RESULTS: ZNC(C) PR-treated C6 conditioned medium increased the ratio of neurite-bearing PC12 cells by 36 % compared to the untreated C6 conditioned medium or to a mixture of ZNC (C) PR with the untreated C6 conditioned medium. RBA showed a specific binding site of ZNC(C)PR on C6 cells with Kd value of 2.74 nmol·L-1 and Bmax value of 19 pmol·g -1 protein. CONCLUSION: ZNC (C) PR enhanced C6 cells induced secretion of some neurotrophic factors which acted as enhancers for PC12 cells differentiation, through its specific receptor sites on the neu-roglioma cell.目的:研究神经肽ZNC(C)PR通过C6细胞发挥的神经营养作用和机制。方法:以ZNC(C)PR处理C6细胞的条件培养液对PC12细胞生长的影响来观察该肽的间接营养作用,并用受体结合分析来确证该肽在此系统中作用的靶细胞类型。结果:ZNC(C)PR处理的C6细胞的条件培养液能够促进PC12细胞由嗜铬瘤细胞向交感神经元转化;放射性配体分析进一步指出在C6细胞上存在着该肽的特异性结合位点。结论:ZNC(C)PR能够通过位于C6细胞上的受体促进该细胞分泌某种营养性因子,通过这种因子促进PC12细胞的发育。

关 键 词：ARGIPRESSIN C6 cells binding sites PC12 cells cell differentiation conditioned culture media radioligand assay NEURITES 

分 类 号：R96[医药卫生—药理学]