Effect of melatonin on pro-opiomelanocortin mRNA expression in arcuate nucleus of rat hypothalamus  

褪黑素对大鼠下丘脑弓状核前阿黑皮素mRNA表达的影响(英文)

作　　者：俞昌喜[1] 吴根诚[1] 许绍芬[1] 陈崇宏[2] 

机构地区：[1]上海医科大学神经生物学教研室医学神经生物学国家重点实验室 [2]福建医科大学药理学教研室

出　　处：《Acta Pharmacologica Sinica》2000年第5期39-42,共4页中国药理学报（英文版）

基　　金：Project supported by the Natural Science Foundation of Fujian Province of China (№ C96038);the Key Project for the Ninth Five-year Plan of China (№ 96-906-11-01).

摘　　要：AIM: To observe altered expression of pro-opiome-lanocortin (POMC) mRNA localized in neurons in the arcuate nucleus of rat hypothalamus following exogenous administration of melatonin. METHODS: The experimental animals were divided into two groups injected ip with melatonin 90 mg·kg-1 and vehicle at 12 h interval (9:00 am and 9:00 pm). Twelve hours after, the last injection, the rat brains were processed for coronal sections and nonradioactive in situ hybridization histochem-istry technic was used. The integral optical density (IOD) and mean optical density (OD) of the stained brain sections were measured using the computer-assisted image processing technique. RESULTS: The POMC mRNA expression in the arcuate nucleus showed obvious enhancement in the brain sections of rats treated with melatonin. The IOD and OD values in the hypothalamic arcuate nucleus area were increased significantly (P < 0.01, P <0.05 respectively) with melatonin treatment. CONCLUSION: Melatonin may enhance the POMC mRNA expression in the arcuate nucleus of rat hypothalamus.目的:观察外源性给予褪黑素对大鼠下丘脑弓状核内神经细胞的前阿黑皮素(POMC) mRNA表达水平的影响。方法:实验大鼠分给药组及对照组,给药组大鼠间隔12 h腹腔注射褪黑素2次(9∶00 am,9∶00pm),每次剂量90mg·kg^(-1),对照组大鼠注射配药液。最后一次注射后12 h,灌注取脑、冰冻切片,进行原位杂交实验,计算机图像处理技术测定染色脑片积分光密度(IOD)值、平均光密度(OD)值。结果:给药组大鼠下丘脑弓状核内POMC mRNA表达明显增强;其IOD和OD值均显著增加。结论:褪黑素可加强大鼠弓状核内POMC mRNA的表达。

关 键 词：MELATONIN PRO-OPIOMELANOCORTIN messenger RNA arcuate nucleus in situ hybridization computer-assisted image processing ANALGESIA 

分 类 号：R96[医药卫生—药理学]