Effects of XW630 on cell proliferation, iNOS activity, and cGMP content in human osteoblast-like cell line TE85  

XW630对人的类成骨细胞株TE85细胞增殖、iNOS活性和cGMP含量的影响(英文)

作　　者：孙兰[1] 翁玲玲[2] 郑虎[2] 刘景生 

机构地区：[1]中国医学科学院基础医学研究所 [2]华西医科大学药学院药物化学室

出　　处：《Acta Pharmacologica Sinica》2000年第3期71-74,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39430120.

摘　　要：AIM: To study the effects of 2-[3-estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) in human os-teoblast-like cell line TE85. METHODS: [3H]Thymi-dine incorporation and cell count for cell proliferation, ra-dioimmunoassay for cyclic GMP (cGMP) content, and monitoring the conversion of [3H]arginine for inducible nitric-oxide synthase (iNOS) activity assay. RESULTS: After treatment with XW630 for 48 h, [3H]thymidine incorporation and cell numbers increased by 62.7% and 96.9%, respectively. NG-mono-methyl-L-arginine (L-NMMA, an NOS inhibitor) induced a concentration-dependent inhibitory effect on the proliferation after treatment for 48 h The inhibitory effect was prevented partially by XW630 (1.0 nmol·L-1). After treatment with XW630 for 12 -48 h, iNOS activity and cGMP concentration increased in time-dependent manners. CONCLUSION: XW630 stimulated cell proliferation, enhanced iNOS activity and cGMP content in human osteoblast-like cell line TE85.目的:研究XW630对人的类成骨细胞株TE85细胞功能的影响。方法:[~3H]胸腺嘧啶掺入法和细胞计数法测定细胞增殖、放射免疫法测定cGMP含量、精氨酸转化法测定iNOS活性。结果:在培养的人的类成骨细胞中,XW630作用48h后,细胞数增加96.9％,[~3H]胸腺嘧啶掺入量增加62.7％。NOS抑制剂L-NMMA(0.25,0.5,1.0 mmol·L^(-1))作用48h,可剂量依赖地抑制细胞增殖,这一作用可被XW630 1.0nmol·L^(-1)部分阻断。作用时间在30min内,XW630对细胞iNOS活性及cGMP含量无影响。延长作用时间,XW630时间依赖地增加细胞iNOS活性及cGMP含量。结论:XW630刺激人的类成骨细胞增殖,增加细胞iNOS活性和cGMP含量。

关 键 词：XW630 estrogens TETRACYCLINES osteoblasts THYMIDINE cell count RADIOIMMUNOASSAY nitric-oxide synthase cyclic GMP 

分 类 号：R96[医药卫生—药理学]