Metabolism of ginsenoside Rg_1 by intestinal bacteria Ⅱ. Immunological activity of ginsenoside Rg_1 and Rh_1  

人参皂苷Rg_1的肠内菌代谢Ⅱ 人参皂苷Rg_1和Rh_1免疫活性(英文)

作　　者：王毅[1] 王本祥[1] 刘铁汉[2] 南陆彦[3] 永田年 池岛乔[1] 

机构地区：[1]长春中医学院新药研究中心 [2]沈阳药科大学 [3]横滨市立大学医学部寄生虫教研室 [4]滨松医科大学微生物教研室

出　　处：《Acta Pharmacologica Sinica》2000年第9期26-30,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, No 395708612.

摘　　要：AIM: To compare the effect of ginsenoside Rg1 and its metabolite Rh, on proinflammatory cytokines and their mRNA expression by THP-1 cells. METHODS: Hu-man peripheral blood mononuclear cells (PBMC) were incubated with Rg1 and Rh1 at concentrations of 0.1, 1, 10, and 100 mg/L, and the cell proliferation was mea-sured 24 h after incubation. Radioimmunoassay (RIA) was used to detect the production of proinflammatory cy-tokines, TNFα, IL-1α, and IL-8. TNFα mRNA level was detected by reverse transcription polymerase chain re-action (RT-PCR) after administration of Rg1 and Rh1. RESULTS: Rg1 and Rh1(at concentration of 0.1, l, 10, 100 mg/L) had no effect on PBMC proliferation. Rh1 1 mg/L could upregulate the productions of TNF (and IL-8 induced by lipopolysaccharides (LPS) 10 mg/ L plus phorbol myristate acetate (PMA) 200 nmol/L, however, Rg1 showed an inhibitory effect on TNFα pro-duction induced by LPS 100 mg/L. Rg1 1 mg/L and Rh1, 100 mg/L enhanced the production of IL-lα level in THP-1 cells in the presence of LPS 10 mg/L. RT-PCR revealed that Rh1 stimulated TNFα mRNA expression in suitable stimulatory conditions. CONCLUSION: Rg1 and Rh1 have different effects on the production of cy-tokines produced THP-1 cells stimulated by LPS and PMA.目的:比较人参皂苷Rg_1及其代谢产物Rh_1对细胞因子及其mRNA表达的影响.方法:将Rg_1及Rh_1加入正常人外周血单核细胞(PBMC)培养24小时后,计数细胞,观察其对正常细胞增殖的影响.用放射免疫法(RIA)观察Rg_1及Rh_1对人组织瘤细胞(THP-1)分泌与炎症有关的细胞因子(IL-1α,TNFα,IL-8)产生的影响,用逆转录酶链式聚合反应(RT-PCR)方法,检测Rg_1及Rh_1对TNFα的mRNA表达的影响.结果:Rg_1及Rh_1(0.1,1,10,100 mg/L)对PBMC增殖无明显影响.但在脂多糖10 mg/L和PMA 200 nmol/L存在下,Rh_1的低浓度(1 mg/L)能促进THP-1细胞产生TNFα与IL-8.而Rg_1在LPS 100 mg/L时抑制TNFα的产生.Rg_1 1 mg/L及Rh_1 100 mg/L均能促进IL-1α的产生.RT-PCR实验结果表明,Rh_1能显著促进TNFα mRNA的表达.结论:Rg_1与Rh_1的免疫活性有所不同,在有些方面甚至相反.

关 键 词：GINSENG SAPONINS CYTOKINES RADIOIMMUNOASSAY reverse transcriptase polymerase chain reaction 

分 类 号：R285[医药卫生—中药学]