Inhibitory effects of purified green tea epicatechins on contraction and proliferation of arterial smooth muscle cells  

绿茶儿茶酚抑制血管收缩及动脉平滑肌细胞增生(英文)

作　　者：陈振宇[1] 罗伟业 姚晓强[1] 刘志伟[1] 何国强[1] 黄聿[1] 

机构地区：[1]香港中文大学医学院生物化学及生理系

出　　处：《Acta Pharmacologica Sinica》2000年第9期69-74,共6页中国药理学报（英文版）

摘　　要：AIM: The present study was aimed to examine the va-sorelaxant and antiproliferative responses to purified green tea epicatechin mixture, ( - )epicatechin and ( - )epigal-locatechin gallate on rat arterial smooth muscle cells. METHODS: Changes in isometric force were measured by Grass force transducer and effects on cell proliferation were evaluated using [3H] thymidine incorporation assay. RESULTS: Epicatechin mixture, ( - ) epicatechin and ( - )epigallocatechin gallate, which we isolated and puri-fied from jasmine green tea, concentration-dependently, reduced the contractile response to phenylephrine in rat isolated aortic rings with ( - )epigallocatechin gallate be-ing more effective. These three agents also inhibited [3H] thymidine incorporation into DNA in cultured rat aortic smooth muscle cells in a concentration-dependent manner. CONCLUSION: The purified epicatechin derivatives from jasmine green tea relaxed the isolated rat arteries preconstricted by phenylephrine and inhibited aor-tic smooth muscle cell proliferation.目的:本文研究从绿茶分离提纯儿茶酚衍生物的血管舒张和抗平滑肌细胞增生的作用.方法:测定分离的大白鼠动脉及肠系膜动脉的收缩力以及动脉平滑肌增生能力.结果:儿茶酚混合物以及其中两个衍生物(表儿茶酚和没食子表食子儿茶酚)浓度依赖性地舒张去甲肾上腺素收缩的动脉以及抑制血管平滑肌细胞增生.结论:绿茶分离提纯儿茶酚混合物及其中两个衍生物具有血管舒张的功能.而它们抗血管平滑肌细胞增生的作用更为显著.没食子表食子儿茶酚是绿茶儿茶酚混合物的主要成份,它的药理作用明显强过表儿茶酚.

关 键 词：tea CATECHOLS PROLIFERATION vasodila-tion smooth muscle ARTERIES 

分 类 号：R96[医药卫生—药理学]