Effects of polysaccharides (FI_0-b) from mycelium of Ganoderma tsugae on proinflammatory cytokine production by THP-1 cells and human PBMC (I)~1  

松杉灵芝菌丝体多糖(FI_0-b)对人组织瘤细胞和人外周血白细胞产生炎症性细胞因子的影响(Ⅰ)(英文)

作　　者：高晓霞[1] 费晓方[1] 王本祥[1] 张洁[2] 宫毓静[1] 南陆彦[3] 永田年 池岛乔[1] 

机构地区：[1]长春中医学院新药研究中心 [2]长春中医学院中药系分析化学教研室 [3]横滨市立大学医学部寄生虫学教研室 [4]滨松医科大学微生物学教研室

出　　处：《Acta Pharmacologica Sinica》2000年第12期124-130,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, No 39077847, Japan China Medical Assocition and Human Science Foundation.

摘　　要：AIM: To compare the effects of water-soluble poly sac -charides, FI0-b, and its formic acid-modified derivative, FI0-b-H, on production of human proinflammatory cy-tokines. METHODS: The polysaccharides were modified by formic acid. Cytokine production was quantitat-ed by radioimmunoassay. mRNA for cytokines was measured by semi-quantitative RT-PCR. RESULTS: FI0-b and FI0-b-H 4, 40, and 400 mg/L significantly downregulated interleukin-1α (IL-1α) production by THP-1 cells induced by lypopolysaccharide (LPS) 1 or 10 mg/ L and phorbol myristate acetate (PMA) 200 nmol/L. At lower stimulation with LPS 10 mg/L and PMA 200 nmol/L, both polysaccharides significantly upregulated tumor necrosis factor α (TNFα) production by THP-1 cells. However, at higher stimulation with LPS 100 mg/L and PMA 200 nmol/L, they downregulated TNFα production. FI0-b-H downregulated interleukin-8 (IL-8) production by THP-1 cells at a lower-dose of LPS 1 mg/ L and PMA 200 nmol/L, but upregulated IL-8 production at a higher-dose of LPS 10 mg/L and PMA 200 nmol/L. Production of cytokines (IL-lα and TNFα)was transcriptionally or post-transcriptionally regulated by FI0-b and FI0-b-H. CONCLUSION: The water-soluble polysaccharides of Ganoderma tsugae mycelium have bidirectional immunomodulatory effects on cytokine production in different stimulatory conditions in a dose-dependent manner. Compared with FI0-b, FI0-b-H has more marked effects on human proinflammatory cytokine production.目的:比较研究松杉灵芝水溶性多糖(Fl_0-b)及其甲酸修饰产物(FI_0-b-H)对人炎症性细胞因子产生的影响.方法:用放射免疫分析法(RIA)及逆转录聚合酶链反应(RT-PCR)分析法研究FI_0-b和Fl_0-b-H对人组织瘤细胞(THP-1)和人外周血单核细胞(PBMC)分泌的各种与炎症有关的细胞因子(白介素-1 α,6,8,肿瘤坏死因子α)及对白介素-1α,肿瘤坏死因子αmRNA表达的影响.结果:FI_0-b和FI_0-b-H(浓度分别为4,40,400 mg/L)显著抑制LPS 10 mg/L协同PMA 200 nmol/L诱导的THP-1细胞和PBMC细胞产生IL-1α和TNF α的量.当低浓度刺激剂(LPS 1mg/L协同PMA 200 nmol/L)刺激THP-1细胞时,FI_0-b-H明显提高白介素-8的产生;但当高浓度刺激剂(LPS 10 mg/L协同200 nmol/L PMA)刺激THP-1-细胞时,FI_0-b-H却明显抑制白介素-8的产生.FI_0-b-和FI_0-b-H对THP-1细胞白介素-1α和肿瘤坏死因子α的mRNA表达有显著作用.结论:与FI_0-b相比,FI_0-b-H在人炎症性细胞因子的产生方面表现出较强的活性.松杉灵芝菌丝体水溶性多糖在不同的刺激条件下,具有双向免疫调节作用,并具有一定的剂量依赖关系.

关 键 词：Ganoderma tsugae POLYSACCHARIDES INTERLEUKIN-1 INTERLEUKIN-6 INTERLEUKIN-8 tumor necrosis factor 

分 类 号：R285[医药卫生—中药学]