Prostaglandins but not nitric oxide are endothelium-derived relaxing factors in the trout aorta  

前列腺素而非一氧化氮是鳟主动脉内皮细胞舒血管因子(英文)

作　　者：Virginia M MILLER Paul M VANHOUTTE 

机构地区：[1]Institut De Recherches Internationales Servier,6,place des Pléiades 92415 Courbevoie Cedex,France

出　　处：《Acta Pharmacologica Sinica》2000年第10期10-15,共6页中国药理学报（英文版）

基　　金：Project supported in part by grants from the National Institutes of Health HL 31183;the Mayo Clinic and Foundation.

摘　　要：AIM:To identify the type of prostanoids produced by endothelial cells of trout aorta and to determine whether or not the smooth muscle responds to nitric oxide. METHODS:Ventral aortas, with and without endotheli-um from rainbow trout (S gairdneri), were incubated in a buffered salt solution. RESULTS:Addition of the calcium ionophore A23187 caused a significant increase in prostaglandin E's and a consistent increase in the stable metabolite of prostacyclin (6-keto-prostaglandin F1a) in the incubation media only when the endothelium was present. This production was inhibited by methylene blue (10umol/L). In rings of trout aorta without endothelium suspended for the measurement of isometric force in organ chambers,prostacyclin and prostaglandin E1 but not prostaglandin E2 caused concentration-dependent decreases in tension when the rings were contracted with acetyl-choline. The smooth muscle did not relax to nitric oxide but did so to sodium nitroprusside. Relaxations to the latter nitrovasodilator were not inhibited by methylene blue. Descending aorta without endothelium from frogs relaxed in a concentration-dependent manner to nitric oxide. CONCLUSION:Predominant endothelium-de-rived relaxing factors in trout aorta are prostaglandins, the synthesis of which can be inhibited by methylene blue.A phylogenetic appearance of nitric-oxide sensitive mechanism for vasodilatation, perhaps is associated with the transition from water to air respiration.AIM:To identify the type of prostanoids produced by endothelial cells of trout aorta and to determine whether or not the smooth muscle responds to nitric oxide. METHODS:Ventral aortas, with and without endotheli-um from rainbow trout (S gairdneri), were incubated in a buffered salt solution. RESULTS:Addition of the calcium ionophore A23187 caused a significant increase in prostaglandin E’s and a consistent increase in the stable metabolite of prostacyclin (6-keto-prostaglandin F1a) in the incubation media only when the endothelium was present. This production was inhibited by methylene blue (10umol/L). In rings of trout aorta without endothelium suspended for the measurement of isometric force in organ chambers,prostacyclin and prostaglandin E1 but not prostaglandin E2 caused concentration-dependent decreases in tension when the rings were contracted with acetyl-choline. The smooth muscle did not relax to nitric oxide but did so to sodium nitroprusside. Relaxations to the latter nitrovasodilator were

关 键 词：ACETYLCHOLINE calcium IONOPHORES Rana catesbiana methylene blue 

分 类 号：R33[医药卫生—人体生理学]