Effect of histamine H3-receptor antagonist clobenpropit on spatial memory of radial maze performance in rats  

组胺H_3受体拮抗剂clobenpropit对大鼠放射状迷宫程序空间记忆的作用(英文)

作　　者：陈忠[1] 

机构地区：[1]浙江大学医学院基础医学系药理学研究室

出　　处：《Acta Pharmacologica Sinica》2000年第10期44-49,共6页中国药理学报（英文版）

摘　　要：AIM:The effect of histamine H3-receptor antagonist, clobenpropit (VUP9153) on spatial memory deficits induced by scopolamine was investigated in rats. METHODS:Eight-Arm radial maze performance was used to measure spatial memory in rats, and the brain regions were subsequently dissected and histamine contents were determined by HPLC.RESULTS:Intracerebroven-tricular(icv) injection of clobenpropit (50μg) ameliorated memory impairment induced by scopolamine in both parameters of radial maze performance. The amelioration induced by clobenpropit was antagonized by an H3-agonist,(R)-a-methylhistamine (5-10μg).a-Fluo-romethylhistidine (10, 20μg), a histidine decarboxylase inhibitor, also effectively reversed clobenpropit-induced ameliorating effects. CONCLUSION:The brain histamine H3-receptor antagonists are highly related to the spatial memory, and this action may be due to cholinergic neurons.目的:研究中枢组胺H_3受体拮抗剂clobenpropit{S-[3-(4(5)-咪唑基)-丙基-N-(4-氯苄基)]异硫脲,VUF9153}对东莨菪碱引起的大鼠空间记忆障碍的作用.方法:采用8-方向放射状迷宫的程序研究大鼠的空间记忆,并利用高效液相法测定脑内组胺含量.结果:侧脑室内注射clobenpmpit(50μg)可明显对抗东莨菪碱导致的空间记忆障碍.相反,组胺H_3受体激动剂(R)-α-甲基组胺(5,10μg)可明显抑制clobenpropit的空间记忆改善作用.另外,clobenpropit的作用被α-氟甲基组胺酸(10,20μg)有效地抑制.结论:中枢组胺H_3受体与大鼠空间记忆的调节密切相关,而且其作用与胆碱能神经相关.

关 键 词：memory disorder SCOPOLAMINE his-tamine H3-receptors CLOBENPROPIT 

分 类 号：R96[医药卫生—药理学]