Tripterygium wilfordii saponins and interleukin-10 prevent induction of experimental autoimmune thyroiditis by dendritic cells  

雷公藤皂苷和白介素-10抑制树突状细胞诱导实验性自身免疫性甲状腺炎(英文)

作　　者：王胜军[1] 许化溪[1] 刘恭植[1] 

机构地区：[1]镇江医学院免疫学研究室

出　　处：《Acta Pharmacologica Sinica》2000年第10期58-62,共5页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation or China, № 339370364;Scientific Research Foundation of Sceince and Technology Committee of Jiangsu.

摘　　要：AIM:To study the roles of Tripterygium wilfordii saponins (TⅡ) and interleukin-10 (IL-10) on dendritic cells (DC)-induced experimental autoimmune thyroiditis (EAT). METHODS:We used mice as autoimmune thyroiditis model animals and divided them into 4 groups, namely DC group, Tn group, IL-10 group, and control group. The level of thyroglobulin (Tg) antibody was assayed by ELISA. TNFB production in the cultured su-pernatants and nitric oxide (NO) in the serum were measured by biological activation assay and Griess reaction, respectively. Tg-stimulated proliferation of lymphocytes was detected with MIT incorporation assay. The histopathological analysis of thyroid was carried out. RESULTS: Tg-pulsed DC were able to induce EAT with increase in the concentration of TgAb in serum and lymphocytes infiltration in thyroid. After treatment with TⅡ or IL-10, DC could not induce EAT with lower levels of TgAb and no lymphocyte infiltration. The concentration of NO in serum, TNFB activation, and the proliferation of lymphocytes in response to thyroglobulin in TⅡ or IL-10 group were lower than those in DC group. CONCLUSION: TⅡ and IL-10 are able to strongly inhibit the ability of DC to induce experimental autoimmune thyroiditis .目的:研究雷公藤皂苷和白介素-10(IL-10)在树突状细胞(DC)诱导实验性自身免疫性甲状腺炎(EAT)中的作用.方法:ELISA法检测甲状腺球蛋白抗体水平,TNF_β和NO分别采用生物学方法及Griess法测定,淋巴细胞增殖试验采用MTT掺入法.结果:DC能诱导EAT的发生,表现为甲状腺内出现淋巴细胞浸润并伴有血清中TgAb明显升高;但经雷公藤皂苷和IL-10处理后,DC不能诱导EAT的发生,表现为甲状腺无病理改变,血清中TgAb明显低下.同时,雷公藤皂苷组和IL-10组小鼠TNF_β活性、NO浓度和淋巴细胞增殖能力显著低于树突状细胞组(P<0.05).结论:雷公藤皂苷和IL-10能显著抑制DC诱导EAT的发生.

关 键 词：Tripterygium wilfordii SAPONINS in-terleukin-10 dendritic cells AUTOIMMUNITY THYROIDITIS nitric oxide tumor necrosis factor 

分 类 号：R96[医药卫生—药理学]