肝炎肝硬变患者肝细胞凋亡及其基因调控的检测  

THE INVESTIGATION OF HEPATOCYTE APOPTOSIS AND ITS MODULAT- ING FACTORS IN CIRRHOTIC LIVER TISSUES

作　　者：王敬文[1] 施宝民[2] 陶得定[2] 杨镇[2] 

机构地区：[1]山东潍坊医学院附属青州医院肝胆外科,山东省青州市262500 [2]同济医科大学附属同济医院普外科

出　　处：《实用肝脏病杂志》2000年第1期6-8,共3页Journal of Practical Hepatology

摘　　要：目的 研究肝炎肝硬变患者肝细胞的死亡方式及其相关调控因素。方法 选20例肝炎肝硬变患者手术所取肝组织,以光镜、电镜、原位末端标记法(TUNEL)和流式细胞仪检测肝细胞凋亡;并以免疫组化法检测凋亡的相关调控基因Bcl-2,C-myc,C-fos和细胞因子,TGFβ,TNFα、iNOS。结果 肝炎肝硬变患者肝细胞有凋亡和坏死两种死亡模式,与对照组相比凋亡数量显著增加,凋亡指数分别为3.06+/-0.79％,0.56+/-0.2％(P<0.05)。免疫组化法检测Bcl-2,C-myc,C-fos,TGFβ、TNFα、iNOS的蛋白质表达,肝炎肝硬变者均为阳性或强阳性;正常肝为阴性。结论 凋亡是肝炎肝硬变肝细胞死亡模式之一,而且其凋亡数量增加与Bcl-2,C-myc,C-fos,TGFβ、TNFα、iNOS的高量表达有关。Objective To investigate the death patterns of hepatocyte in patients with cirrhosis. Methods 20 liver specimens of cirrhostic patients were collected during operation and other 5 controls from patients with liver he-mangioma. Hepatocyte apoptosis were identified under light microscopy,electric microscopy and measured by flow cy-tometry.The affecting factors of apoptosis such as Bcl-2,C-myc,C-fos,TGFβ,TNFα、iNOS were also estimated by immunohistochemistry. Results There were two different death patterns simultaneously in these cirrhotic tissues: apoptosis and necrosis. The amount of apoptosis remarkably increased in cirrhosis group with comparison to that of controls with the apoptosis index of 3.06 +/-0.79%to 0.56+/-0.20%,respectively( P < 0.05) .The expressions of Bcl-2,C-myc,C-fos,TGFβ,TNFα,iNOS were positive or densely positive in cirrhotic group,while negative in normal liver tissues. Conclusion Apoptosis is one kind of death patterns in liver cirrhosis due to viral hepatitis and plays an important role in the pathogenesis of cirrhosis. The apoptosis may be related to certain genes and cytokines such as Bcl-2,C-myc,C-fos and TGFβ,TNFα、 iNOS.

关 键 词：肝炎肝硬变 凋亡 细胞癌基因 

分 类 号：R575.2[医药卫生—消化系统]