帕金森病大鼠模型多巴胺转运蛋白^(125)I-β-CIT放射自显影研究  

Autoradiographic study of dopamine transporter in rat models of Parkinson disease with ^(125)I-β-CIT

作　　者：刘振国[1] 陈生弟[1] 孙文善[2] 沈鸣华[3] 李培勇[2] 

机构地区：[1]上海第二医科大学附属瑞金医院神经科、帕金森病诊疗与研究中心 [2]上海第二医科大学附属瑞金医院核医学科 [3]上海医科大学放射药物教研室

出　　处：《中华神经科杂志》2000年第4期7-9,64,共4页Chinese Journal of Neurology

基　　金：国家重点基础研究规划"脑功能和脑重大疾病基础研究"资助项目(G1999054000);上海市卫生系统百名跨世纪优秀学科带头人培养计划资助项目(97BR001);上海市科委青年科技"启明星"计划资助项目(97QMB1410、99QB14023);上海市教委基金资助项目(99B08)

摘　　要：目的　探讨1 2 5 I β CIT[甲基 3β (4 碘苯基 )托烷 2 β 羧酸盐 ]多巴胺转运蛋白 (DAT)显像技术的临床应用价值。方法　应用 6 羟基多巴胺 (6 OHDA)建立黑质部分损毁及完全损毁偏侧帕金森病 (PD)大鼠模型。静脉注射1 2 5 I β CIT 40 μCi进行放射自显影。高效液相 电化学法检测纹状体多巴胺 (DA)及其代谢产物含量。免疫组化酪氨酸羟化酶 (TH)染色观察黑质及纹状体TH阳性细胞及纤维。结果　黑质部分损毁及完全损毁偏侧模型损毁侧纹状体1 2 5 I β CIT放射活性分别为 2 .6 7± 0 .2 5和 0 .98± 0 .2 9,较未损毁侧分别降低 18%和 72 % ,DA含量分别降低 39%和 98%。TH染色可见损毁侧黑质及纹状体TH阳性细胞及纤维明显少于对侧。结论　DAT的显像研究可能有助于PD早期诊断及病情监测。Objective To study the value of imaging for dopamine transporter(DAT) with 125 I β CIT. Methods The rat models of hemiparkinsonism with either partial or complete lesions were rendered with 6 hydroxydopamine (6 OHDA). Each rat was injected intravenously with 125 I β CIT containing 40μCi. Coronal tissue sections were imaged by autoradiography. The levels of dopamine (DA) and its metabolites were measured by high performance liquid choromatography and electrochemical detection (HPLC ECD). The tyrosine hydroxylase(TH) positive cells and fibres in the substantia nigra and striatum of the rats were observed with immunohistochemical staining. Results The radioactivities in the lesions of the striatum of both partial and complete hemiparkinsonian lesions of the rat models were 2.67±0.25 and 0.98±0.29,respectively ,and were singificantly decreased by 18% and 72%,respectively, as compared with those on the side without lesions. The levels of DA in the lesions of the striatum of partial and complete rat models were decreased by 39% and 98%, respectively. The loss of TH positive cells and fibres in the substantia nigra and striatum was found in the lesions of the striatum of both partial and complete hemiparkinsonian rat models. Conclusion The imaging study of DAT may be helpful for the early diagnosis of Parkinson′s disease and for the monitoring of the progression of this disease.

关 键 词：载体蛋白质类 帕金森病 ^(125)I-β-CIT 放射自显影术 

分 类 号：R742.5[医药卫生—神经病学与精神病学]