La^(3+) Transmembrane Research in Guinea Pig Ventricular Cells by Fura-2 Fluorescence  

作　　者：Rong CHEN, Pin YANG, Chun Ying WEI (Institute of Molecular Science, Shanxi University. Taiyuan 030006) 

出　　处：《Chinese Chemical Letters》2000年第8期725-726,共2页中国化学快报（英文版）

摘　　要：Binding of La3+ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La3+ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1 mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La3+can not enter guinea pig ventricular cells in this case.Binding of La3+ to Fura-2 can change 340/380 nm fluorescence intensity ratio. Whether La3+ cross ventricular cell membrane was detected by this fluorescent probe technique. Fura-2 loaded isolated guinea pig ventricular cells were exposed to 0.01-0.1 mM extracellular Lanthanum ion concentration, 340nm/380 nm fluorescence ratio was not changed. Using calcium channel agonist BAY K8644, KCL (35mM) depolarization to open the voltage-dependent calcium channel (VDCC); Adrenoceptor agonist to excite adrenoceptor, 340/380 ratio was not changed, suggesting that La3+can not enter guinea pig ventricular cells in this case.

关 键 词：Ventricular cells ADRENALINE La3+ TRANSMEMBRANE FURA-2 BAY K8644. 

分 类 号：O614[理学—无机化学]