皮革废物铬革屑的微生物学处理研究Ⅱ:降解革屑微生物的发酵条件及其蛋白酶的降解研究  

STUDY ON MICROBIAL TREATMENT OF LEATHER WASTEⅡ: MICROBIAL FERMENTATION CONDITIONS FOR DEGRADATION OF LEATHER WASTE AND HYDROLYSIS OF LEATHER WASTE BY PROTEASE

作　　者：董燕[1] 黄秀梨[1] 

机构地区：[1]北京师范大学生命科学学院,北京100875

出　　处：《应用与环境生物学报》1999年第S1期123-126,共4页Chinese Journal of Applied and Environmental Biology

摘　　要：在以皮胶原为碳、氮源时其最适产酶条件为ｐ Ｈ７ ，３０ ℃，高产酶时间为４ ｄ ．经 Ｃａ（ Ｏ Ｈ）２ 预处理革屑不能有效促进该菌对革屑的降解，但可使大部分 Ｃｒ３ ＋ 沉淀而除去．经该株蛋白酶的粗提及性质实验，确定酶的最适反应条件为４０ Ｃ、ｐ Ｈ７ ．０ ～８ ．０ ， Ｃｒ３ ＋ 对酶有轻度的抑制作用，以２ ．２６ ％ 的粗酶作用于革屑，水解率达５０ ％When leather waste was used as C and N sources, the optimum fermentation conditions were: initial pH 7.0, temperature 30 ℃and culture times 4 d. The pretreatment with Ca(OH) 2 could not promote the degradation, but it could remove most of Cr 3+ . The protease from P. raciborskii AS 3.1066E was purified by (NH 4) 2SO 4 precipitation, and its maximum activity was found at pH 7.0～8.0 and temperature 40 ℃. The protease was slightly inhibited by Cr 3+ . The conversion rate of leather waste was about 50% at the presence of 2.26% crude enzyme.

关 键 词：铬革屑 皱绒青霉AS3.1066E 蛋白酶 

分 类 号：X172[环境科学与工程—环境科学]