Fe^(3+)、Mg^(2+)对跨界融合子Foaz中β-类胡萝卜素水平的影响  

EFFECTS OF Fe^(3+) AND Mg^(2+) ON THE LEVELS OF β -CAROTENE IN INTER-KINDOM FUSANT CELL *

作　　者：鲁鹏[1] 程攻[2] 杨兴烨[1] 程树培[1] 

机构地区：[1]南京大学环境科学与工程系污染控制与资源化国家重点实验室,南京210093 [2]南京大学生物化学系,南京210093

出　　处：《应用与环境生物学报》1999年第S1期175-177,共3页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金;江苏省自然科学基金

摘　　要：测定了由光合细菌 Ｐ９４７９ 株与酵母 Ｙ９４０７ 株经原生质融合构建的跨界融合子 Ｆｏａｚ 及其亲株在净化豆制品废水反应中０ 、０ ．１ 、５ ．０ 、１０ ．０ 、１００ ．０ ｍｇ／ Ｌ Ｆｅ３ ＋ 质量浓度和０ 、１５０ 、３００ 、４００ 、８００ ｍｇ／ Ｌ Ｍｇ２ ＋ 质量浓度下的β－类胡萝卜素色素含量及 Ｂ Ｏ Ｄ５ 去除效率．结果表明， Ｆｅ３ ＋ 对融合子 Ｆｏａｚ 及光合细菌中β－ 类胡萝卜素的合成有明显的促进作用，当ρ（ Ｆｅ３ ＋ ） 为５ ．０ ｍｇ／ Ｌ 时，融合子 Ｆｏａｚ 及光合细菌中β－ 类胡萝卜素的质量分数ｗ 达到最高水平，分别为４ ．９ ｍｇ／ｇ 和２ ．４ ｍｇ／ｇ ．而 Ｍｇ２ ＋ 对融合子及其亲株中β－The inter-kingdom fusant cell Foaz was constructed from the protoplast fusion between Rhodobacter sphaeroides and Saccharomyces cerevisiae . The levels of β -carotene in the fusant cell and its parantal strains were determined in the reaction solution with different concentrations of Fe 3+ and Mg 2+ . The results showed that Fe 3+ might promote the production of β -carotene for the fusants and R. sphaeroides . Especially, the mass fraction of β -carotene could reach the highest levels which were 4.9 mg/g for the fusant and 2.4 mg/g for R.sphaeroides , respectively, while the mass concentration of Fe 3+ was 5.0 mg/L. But Mg 2+ could inhibit the production of β -carotene in the fusant and its parental strains.

关 键 词：跨界融合子 豆制品废水 Fe³⁺ Mg²⁺ β-类胡萝卜素 

分 类 号：Q562[生物学—生物化学]