红光结合血卟啉单甲醚光动力疗法治疗微静脉畸形的实验研究  

A experimental study of treatment of venular malformations with photodynamic therapy combined Hematoporphyrin Monomethyl Ether with red laser light

作　　者：黄雪蕾[1,2] 林李嵩[3] 杨栋[4] 

机构地区：[1]解放军总医院口腔颌面外科,博士生北京100853 [2]解放军第一七四医院 [3]福建医科大学附属第一医院口腔科,主任主任医师教授福建350005 [4]厦门大学医学院抗癌研究中心,副教授福建361005

出　　处：《口腔颌面修复学杂志》2011年第4期193-196,共4页Chinese Journal of Prosthodontics

摘　　要：目的:通过鸡冠动物模型了解红光结合光敏剂血卟啉单甲醚(hematoporphyrin monomethylether,HMME)在光动力疗法治疗微静脉畸形中的治疗效果和适宜治疗参数。方法:48只鸡随机分组,处理组分别注射不同剂量的HMME后,半导体激光(红光)或氩离子泵浦染料激光不同能量密度分组照射,照射后肉眼和光镜下观察比较组织损伤程度并测量损伤深度。结果:红光结合HMME照射能引起鸡冠颜色变白、血管数量减少等形态学改变。HMME10mg/kg、15mg/kg剂量组中,在相同功率密度时,红光高能量密度照射组(240J/cm2)所造成损伤深度(分别为1.2225±0.8457mm,2.2800±1.3665mm)均显著大于氩离子泵浦染料激光不同能量密度的各照射组(P<0.05)。适宜治疗参数为HMME剂量10mg/kg,红光能量密度120-240J/cm2。结论:红光结合HMME光动力疗法有可能对增厚或结节型微静脉畸形实现更佳的治疗效果。Objective： To initially investigate the effects and application parameters of photodynamic therapy combined red light with hematoporphyrin monomethyl ether（HMME）on venular malformations.Methods： 48 adult chickens were randomly divided into 6 control groups and 12 experiment groups.Experiment groups were injected HMME at 5,10 or 15mg/kg dose,then irradiated by argon ion pumped dye laser or semiconductor laser（red light）.The energy density of the former was 60J/cm2 or 120J/cm2.The latter＇s was 120J/cm2 or 240J/cm2.All of power density were 200mW/cm2.The observation by unaided eyes and light microscopy were conducted after irradiation,including the degree and depth of injury in the combs tissue.Results： The area of irradiated combs in the experiment groups with red light（semiconductor laser of wavelength 630nm） combined with HMME showed blanching and decrease of blood vessel amount.With the same power density and HMME at 10mg/kg or 15mg/kg dose,experiment groups of red light with high energy density（240J/cm2） showed significantly deeper impairment（respectively were 1.2225±0.8457mm,2.2800±1.3665mm） than those groups of argon ion pumped dye laser with different energy density（P 0.05）.The optimal treatment parameter should be recommended as HMME dose of 10mg/kg and red light with energy density between 120-240J/cm2.Conclusions： Photodynamic therapy combined red light with HMME is likely to realize a preferable therapeutic efficacy for nodular or thicken venular malformations.

关 键 词：光动力疗法 微静脉畸形 激光 光敏剂 鸡冠 

分 类 号：R783[医药卫生—口腔医学]