ras突变和rasP21蛋白表达与尿路上皮肿瘤预后关系  

Ras Gene Mutation and Ras P21 Protein Overexpression and DNA Abormality in Urothlial Tumors Correlated with its Prognosis

作　　者：涂响安[1] 赵志毅[1] 黄春南[1] 余明年[1] 冯家骅[1] 刘丽萍[1] 李全贞[2] 左连富[3] 

机构地区：[1]广东省肇庆市第一人民医院泌尿外科,肇庆526021 [2]中山医科大学达安基因诊断中心 [3]河北省肿瘤研究所

出　　处：《现代泌尿外科杂志》1999年第4期193-195,201,共4页Journal of Modern Urology

摘　　要：应用聚合酶链式反应加单链多态性分析(PCR-SSCP)及流式细胞木和细胞免疫荧光技术检测52倒尿路上皮肿瘤K-ras基因突变及rasP21蛋白表达和DNA含量变化,研究ras突变和rasP21蛋白表达及DNA倍体异常与尿路上皮肿瘤预后关系。K-ras基因突变5例(9.65),rasP21蛋白阳性表达36例(69.2％);异倍体22例(42.3％),二倍体30例(57.7％);DNA倍体异常和rasP21蛋白表达随肿瘤病理分级和分期的上升而增加,与肿瘤预后呈正相关(P<0.01);异倍体肿瘤其rasP21蛋白表达率明显高于二倍体肿瘤(P<0.01)。DNA倍体异常和rasP21蛋白表达对尿路上皮肿瘤预后有预测意义。To study K-ras gene mutation and Ras P21 protein expression and DNA ploidy abnormality. and find their correlation with prognosis in 52 cases of urothelial tumors. K-ras gene mutation was detected by polymerse chain reaction and single-stranded comformation polymorphism. Ras P21 and DNA content using flow cytomytry and cellular immunofluosecence technique from 52 cases of urothelial tumors. K-ras mutation was observed in 9. 6% and the positive expression rate of ras P21 protein in 69. 2%. 42.3% aneuploidy and 57. 7% diploidy tumors were detected. DNA ploidy abnormality and the positive expression of ras P21 protein increased well with rise of tumor grade and stage, but had a worse prognosis( P <0. 01). The results indicated that DNA ploidy abnormality and ras P21 protein overexpression have been shown to be siginificant predictors of the prognosis of urothlial tumor.

关 键 词：尿路上皮肿瘤 基因 预后 聚合酶链式反应 流式细胞术 

分 类 号：R737.1[医药卫生—肿瘤]