Structure Analysis of the Complete Mitochondrial Genome in Cultivation Variety ‘Rongfu’  

作　　者：ZHANG Jing LI Ning ZHANG Zhifeng LIU Tao 

机构地区：[1]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,P.R.China

出　　处：《Journal of Ocean University of China》2011年第4期351-356,共6页中国海洋大学学报（英文版）

基　　金：supported by funds of the National Department Public Benefit Research Foundation of China (Nos. 200805075, 200903030);the National Natural Science Foundation of China (No. 40606035);the National Agricultural Transformation (2010GB23600666)

摘　　要：In this report,complete mitochondrial genome sequences of Laminaria cultivation variety 'Rongfu' were obtained.The results showed the length of circular molecule of mtDNA was 37 638 bp(64.7% A+T),encoding three rRNAs(23S,16S and 5S),25 tRNAs,35 known mitochondrial proteins and 3 ORFs.Sequence alignment indicated its mtDNA genome was very similar to that of Laminaria japonica.Phylogenetic trees inferred from concatenated 30 mitochondrial genes showed that 'Rongfu',Laminaria japon-ica,Laminaria longipedalis,Laminaria diabolica,Laminaria religiosa and Laminaria ochotensis clustered together.In addition,compared with mitochondrial genome of L.japonica,'Rongfu' mtDNA lacked a non-coding region of 19 nucleotides,which was located between rRNA small subunit gene 3(rps3)and rRNA small subunit gene 9(rps9).Seven cultivation varieties of China were divided into two groups based on this non-coding region which was absent in 'Rongfu','Fujian' and 'Sanhai' while present in 'Ailunwan','Dongfang No.2','Dongfang No.3' and 'Zaohoucheng'.So this variation can be used in germplasm identification of cultivation variety.In this report,complete mitochondrial genome sequences of Laminaria cultivation variety ＇Rongfu＇ were obtained.The results showed the length of circular molecule of mtDNA was 37 638 bp（64.7% A＋T）,encoding three rRNAs（23S,16S and 5S）,25 tRNAs,35 known mitochondrial proteins and 3 ORFs.Sequence alignment indicated its mtDNA genome was very similar to that of Laminaria japonica.Phylogenetic trees inferred from concatenated 30 mitochondrial genes showed that ＇Rongfu＇,Laminaria japon-ica,Laminaria longipedalis,Laminaria diabolica,Laminaria religiosa and Laminaria ochotensis clustered together.In addition,compared with mitochondrial genome of L.japonica,＇Rongfu＇ mtDNA lacked a non-coding region of 19 nucleotides,which was located between rRNA small subunit gene 3（rps3）and rRNA small subunit gene 9（rps9）.Seven cultivation varieties of China were divided into two groups based on this non-coding region which was absent in ＇Rongfu＇,＇Fujian＇ and ＇Sanhai＇ while present in ＇Ailunwan＇,＇Dongfang No.2＇,＇Dongfang No.3＇ and ＇Zaohoucheng＇.So this variation can be used in germplasm identification of cultivation variety.

关 键 词：LAMINARIA mitochondrial DNA structure analysis phylogenetic relationship germplasm identification 

分 类 号：P[天文地球]