Genetic Differentiation of Japanese Sardinella(Sardinella zunasi) Populations in the Northwest Pacific Revealed by ISSR Analysis  

作　　者：YING Yiping GAO Tianxiang MIAO Zhenqing 

机构地区：[1]Fisheries College,Ocean University of China,Qingdao 266003,P.R.China [2]Marine College,Zhejiang Ocean University,Zhoushan 316000,P.R.China

出　　处：《Journal of Ocean University of China》2011年第4期417-424,共8页中国海洋大学学报（英文版）

基　　金：supported by the Special Research Foundation for Public Welfare Marine Program (200905019-2)

摘　　要：Knowledge of population genetic structure plays an important role in fisheries management.In this research,In-ter-Simple-Sequence-Repeat(ISSR)markers were employed to evaluate the genetic structure of Japanese sardinella(Sardinella zun-asi)populations in the Northwest Pacific.Eighty seven individuals from 5 locations were screened using 4 highly polymorphic prim-ers.A total of 173 polymorphic loci were detected out of 191 loci amplified.Small but significant genetic differentiation was de-tected between the Chinese and Japanese populations by both AMOVA and pairwise FST analyses,which was further supported by cluster analysis.We consider that climate change during glaciations should be responsible for the genetic differentiation.Isolation by geographic distance among populations was observed,indicating that the distance might also lead to the genetic differentiation.However,no genetic structure was found within the populations off both the Chinese and Japanese coasts,indicating a high-level along-coast gene flow,which might result from ocean current transport and common ground for over-wintering.Knowledge of population genetic structure plays an important role in fisheries management.In this research,In-ter-Simple-Sequence-Repeat（ISSR）markers were employed to evaluate the genetic structure of Japanese sardinella（Sardinella zun-asi）populations in the Northwest Pacific.Eighty seven individuals from 5 locations were screened using 4 highly polymorphic prim-ers.A total of 173 polymorphic loci were detected out of 191 loci amplified.Small but significant genetic differentiation was de-tected between the Chinese and Japanese populations by both AMOVA and pairwise FST analyses,which was further supported by cluster analysis.We consider that climate change during glaciations should be responsible for the genetic differentiation.Isolation by geographic distance among populations was observed,indicating that the distance might also lead to the genetic differentiation.However,no genetic structure was found within the populations off both the Chinese and Japanese coasts,indicating a high-level along-coast gene flow,which might result from ocean current transport and common ground for over-wintering.

关 键 词：Sardinella zunasi population genetic differentiation ISSRS 

分 类 号：P[天文地球]