Modulation by Biogenic Amines for the Hemocyte Count and Prophenoloxidase Exocytosis via Receptors in Litopenaeus vannamei  

作　　者：PAN Luqing LIU Huijie YU Jinhong 

机构地区：[1]Department of Aquaculture,the Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,P.R.China

出　　处：《Journal of Ocean University of China》2011年第4期425-432,共8页中国海洋大学学报（英文版）

基　　金：supported by the Program for New Century Excellent Talents in University (No. NCET-06-0597);Ministry of Education 111 Project (No. B08049)

摘　　要：Hemocyte counts and phenoloxidase(PO)activity were examined after hemolymph being incubated in dopamine(DA),noradrenaline(NE)and serotonin(5-HT).Results showed that all the three biogenic amines(BAs)had a significant impact on total hemocyte count(THC),differential hemocyte count(DHC),and intracelluar and extracelluar phenoloxidase(PO)activity.Among these Bas,DA had the strongest effect on the above parameters,whereas 5-HT had the least effect.Preincubation with D1 receptor antagonist SCH23390,D2 receptor antagonist Sulpiride and 1:1 admixture of the two could significantly inhibit the effect of DA on these parameters.SCH23390 showed a stronger inhibitory effect than Sulpiride,and the admixture exhibited the strongest effect.These results suggested that the change of hemocyte count and activation of prophenoloxidase(proPO)system in Litopenaeusvan-namei hemocyte can be regulated by BAs,and DA modulates the two parameters via its receptors.Hemocyte counts and phenoloxidase（PO）activity were examined after hemolymph being incubated in dopamine（DA）,noradrenaline（NE）and serotonin（5-HT）.Results showed that all the three biogenic amines（BAs）had a significant impact on total hemocyte count（THC）,differential hemocyte count（DHC）,and intracelluar and extracelluar phenoloxidase（PO）activity.Among these Bas,DA had the strongest effect on the above parameters,whereas 5-HT had the least effect.Preincubation with D1 receptor antagonist SCH23390,D2 receptor antagonist Sulpiride and 1：1 admixture of the two could significantly inhibit the effect of DA on these parameters.SCH23390 showed a stronger inhibitory effect than Sulpiride,and the admixture exhibited the strongest effect.These results suggested that the change of hemocyte count and activation of prophenoloxidase（proPO）system in Litopenaeusvan-namei hemocyte can be regulated by BAs,and DA modulates the two parameters via its receptors.

关 键 词：biogenic amine dopamine receptor HEMOCYTE Litopenaeus vannamei prophenoloxidase system 

分 类 号：P[天文地球]