Aflatoxin sufferer and p53 gene mutation in hepatocellular carcinoma  

作　　者：DENG Zhuo-Lin MA Yun 

机构地区：[1]不详

出　　处：《World Journal of Gastroenterology》1998年第1期33-34,共2页世界胃肠病学杂志（英文版）

摘　　要：IM To study the p53 gene mutation and its relationship to aflatoxin B1 exposure in hepatocellular carcinoma (HCC).METHODS Restriction fragment length polymorphism analysis method was used in 62 HCC samples, and DNA direct sequencing in another 45 HCC samples.RESULTS In HCC and AFB1 high and lowrisk areas, 36/52 (69%) and 2/10 (20%) cases were found losing the HaeⅢ allele respectively, suggesting one of the base G mutation at the p53 gene codon 249. Similar results appeared in DNA direct sequencing, 20/35 (57%) and 1/10 (10%) respectively mutated at the codon 249 third base G to C transversion.CONCLUSION In HCC after AFB1 exposure, mutation of p53 gene is fixed at codon 249 third base and take the form of G to T transversion. This is a definite marker of mutation which is induced by AFB1 mutagen. It is applicable for molecular epidemiologic survey of the sufferers of AFB1 among HCC cases and for discovering more unknown natural AFB1 contaminated areas..Ａｆｌａｔｏｘｉｎｓｕｆｅｒｅｒａｎｄｐ５３ｇｅｎｅｍｕｔａｔｉｏｎｉｎｈｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａＤＥＮＧＺｈｕｏＬｉｎａｎｄＭＡＹｕｎＳｕｂｊｅｃｔｈｅａｄｉｎｇｓＡｆｌａｔｏｘｉｎＢ１；ｇｅｎｅｓ，ｐ５３；ｍｕｔａｔｉｏｎ；...

关 键 词：Aflatoxin B1 genes p53 MUTATION carcinoma hepatocellular liver neoplasms 

分 类 号：R735.7[医药卫生—肿瘤]