转染IL-2基因的膀胱癌瘤苗诱导的抗肿瘤作用  

Induction of Anti-tumorigenicity by IL-2 Gene-transferred Bladder Cancer Vaccine

作　　者：辛军[1] 王晓雄[1] 孟书礼[1] 王鑫[2] 刘玉晶[1] 刘爽[2] 

机构地区：[1]中国人民解放军总医院泌尿外科,北京100853 [2]中国人民解放军军事医学科学院基础所

出　　处：《现代泌尿外科杂志》1998年第1期1-5,共5页Journal of Modern Urology

摘　　要：为了探讨以白细胞介素2(IL-2)基因修饰的肿瘤细胞作为瘤苗治疗膀胱癌的应用价值,采用脂质体介导法将携带IL-2基因的逆转录病毒载体转染入膀胱癌细胞系BTT_(739)中,流式细胞仪行细胞DNA周期分析,动物实验观察放射线灭活后基因瘤苗(BTT_(739)/IL-2细胞)的抗肿瘤免疫作用。建立能分泌IL-2的膀胱癌瘤苗BTT_(739)/IL-2细胞、DNA周期分析表明IL-2基因的导入及表达对BTT_(739)细胞的增殖无影响。放射线灭活后BTT_(739)/IL-2细胞丧失增殖能力,但仍能维持一定水平的IL-2分泌活性达2周左右。先用灭活的瘤苗免疫小鼠,可诱导免疫保护,3周后接种BTT_(739)时不形成肿瘤;用灭活的瘤苗治疗荷瘤小鼠,可使50％小鼠肿瘤消退并长期存活;对治愈后长期存活的小鼠再次接种高剂量BTT_(739)细胞,仍无肿瘤形成。结果提示转染IL-2基因的膀胱癌瘤苗诱导的抗肿瘤作用对预防肿瘤的发生、抑制和清除微小瘤灶,防止膀胱癌复发具有重要的应用价值。To explore (he use of IL-2 gene-modified tumor cells as vaccine for the treatment of bladder cancer. The retroviral vector carrying IL-2 genes was transfected into murine bladder cancer cell line BTT230 by Lipofectamine. Cell cycle was analysed bv FCM and anti-tumor immunity induced by inactivated gene vaccine was observed in experimental models. The bladder cancer vaccine (BTT739/IL-2 ) auto-secreting IL-2 was established. FCM analysis of cell DNA contents indicated that the IL-2 gene transfer and expression could not influence the proliferation of the gene modified cells. With X-ray irradiation in vitro, growth of the cells was abrogated, but its ability to secrete IL-2 was retained within 2 weeks. The results demanstrated that the antitumor effect of the IL-2 gene-transferred bladder cancer vaccine was claimed to have an important application value in the treatment of bladder cancer.

关 键 词：白细胞介素2 膀胱肿瘤 基因瘤苗 

分 类 号：R737.14[医药卫生—肿瘤]