利用高压微波加热技术提高HCVAG免疫组化检测的效果  

THE USE OF HIGH PRESSURE /MICROWAVE HEATING TECHNIQUES FOR IMPROVING IMMUNOHISTOCHEMICAL ASSAY

作　　者：刘聪[1] 赵连三[1] 周凤[1] 王锦蓉[1] 

机构地区：[1]华西医科大学附一院病毒性肝炎研究室,成都610041

出　　处：《实用肝脏病杂志》1998年第2期85-87,共3页Journal of Practical Hepatology

基　　金：国家自然科学基金(项目号:39670811)资助

摘　　要：目的:应用高压微波加热技术对现行常规免疫组化检测技术进行改进,以提高免疫组化检测的灵敏度。方法:用改进法与常规方法分别对20例肝瘤标本同时进行丙型肝炎病毒抗原(HCVAg)的检测,然后比较其结果。结果与结论:改进法的HCVAg检出率为70％,常规方法的检出率为30％,前者的阳性信号强度明显高于后者,表明高压微波加热免疫组化改进法优于常规方法。High pressure/microwave heating techniques were added to routine protocols for improving sensitivity of immunohistochemical assay.Both the modified and routine protocols were applied for detecting hepatits C virus antigen (HCVAg) in twenty hepatocellular carcinoma samples and their sensitivity was compared.The results showed that there was a higher detection rate (70%) and a very clear background with the new improved protocol than that (30%) with the routine one.

关 键 词：免疫组织化学 高压微波技术 丙型肝炎病毒 

分 类 号：R361[医药卫生—病理学]