DIFFERENTIAL CYTOTOXIC SENSITIVITY AMONG MTT,NR AND ALPASE ACTIVITY ASSAYS IN HUMAN PULP CELLS EXPOSED TO DENTAL RESIN MONOMERS  

作　　者：Jiao Sun(Shanghai Biomaterials Research & Test CenterNinth People＇Hospital,Shanghai Second Medical University,Shanghai 200023,China)Michiyo TAKAKUWA Kazuko SATO and Atsushige SATO(Dept. of Biomaterials Science,Faculty of DentistryTokyo Medical and Denta 

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》1998年第1期37-46,共10页中国生物医学工程学报（英文版）

摘　　要：A transformed human dental pulp cell(LSC) was made to assess thecytotoxicity of dental resin monomers with MTT assay,NR assay and ALPase activity assay. L- 929 cells were used as comparative cell line. The sensitivity amongMTT,NR and ALPase activity assays and between two types of cells were investigated. The relationship of ALPase activity to two colorimetric assays was also evaluated. According to IC50 and statistics analysis on cytotoxicity curve,there were significance differences between MTT and NR assay or both cell lines for TECDMAmonomer (P＜0．005). MTT assay showed lower sensitivity than NR assay in twokinds of cell for both monomer,and LSC cells presented less sensitivity than L-929cells for TEGDMA monomer. The ALPase activity assay revealed the highest sensitivity among the three tested assays. There was a positive relationship between thecontent of ALPase activity and cytotoxic response of the other two assays. The results indicated that the sensitive degree of test assay or cell line may differ from thetest chemicals. MTT assay may be more suitable for screening a wide large of unknown tested materials due to its ralative less viariation with cell lines and moderatesensitivity.A transformed human dental pulp cell(LSC) was made to assess thecytotoxicity of dental resin monomers with MTT assay,NR assay and ALPase activity assay. L- 929 cells were used as comparative cell line. The sensitivity amongMTT,NR and ALPase activity assays and between two types of cells were investigated. The relationship of ALPase activity to two colorimetric assays was also evaluated. According to IC50 and statistics analysis on cytotoxicity curve,there were significance differences between MTT and NR assay or both cell lines for TECDMAmonomer (P＜0．005). MTT assay showed lower sensitivity than NR assay in twokinds of cell for both monomer,and LSC cells presented less sensitivity than L-929cells for TEGDMA monomer. The ALPase activity assay revealed the highest sensitivity among the three tested assays. There was a positive relationship between thecontent of ALPase activity and cytotoxic response of the other two assays. The results indicated that the sensitive degree of test assay or cell line may differ from thetest chemicals. MTT assay may be more suitable for screening a wide large of unknown tested materials due to its ralative less viariation with cell lines and moderatesensitivity.

关 键 词：cytotoxicity hydroxyapatite dental TETRAZOLIUM RESTORATIVE PERIODONTAL CYTOMETRY BALTIMORE screening filling 

分 类 号：R312[医药卫生—基础医学]