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Single-cell electro-mechanical shear flow deformability cytometry
《Microsystems & Nanoengineering》2024年第6期235-247,共13页Junyu Chen Xueping Zou Daniel C.Spencer Hywel Morgan 
The complex structural and molecular features of a cell lead to a set of specific dielectric and mechanical properties which can serve as intrinsic phenotypic markers that enable different cell populations to be chara...
关键词：FLOW DEFORMED ELECTRICAL 
High dimensional proteomic mapping of bone marrow immune characteristics in immune thrombocytopenia
《Science China(Life Sciences)》2024年第8期1635-1647,共13页Feng-Qi Liu Qing-Yuan Qu Ying Lei Qi Chen Yu-Xiu Chen Meng-Lin Li Xue-Yan Sun Ye-Jun Wu Qiu-Sha Huang Hai-Xia Fu Yuan Kong Yue-Ying Li Qian-Fei Wang Xiao-Jun Huang Xiao-Hui Zhang 
supported by the National Natural Science Foundation of China(82230004,81970113,82300149);the National Key Research and Development Program of China(2021YFC2500304);Capital Health Research and Development of Special(2022-1-4082);Peking University Medicine Fund for world's leading discipline or discipline cluster development(71003Y3035).
To investigate the role of co-stimulatory and co-inhibitory molecules on immune tolerance in immune thrombocytopenia(ITP),this study mapped the immune cell heterogeneity in the bone marrow of ITP at the single-cell le...
关键词：immune thrombocytopenia autoimmune diseases co-stimulatory co-inhibitory immune checkpoint CTLA4 Cytometry by Time of Flight bone marrow 
Immune cell pair ratio captured by imaging mass cytometry has superior predictive value for prognosis of non-small cell lung cancer than cell fraction and density
《Cancer Communications》2024年第5期589-592,共4页Jian-Rong Li Chao Cheng 
supported by the Cancer Prevention Research Institute of Texas(CPRIT)(RR180061 to CC);the National Cancer Institute of the National Institute of Health(1R01CA269764 to CC).CC is a CPRIT Scholar in Cancer Research.
Infiltrating immune cells in the tumor microenvironment(TME)play critical roles in the initiation,progression,and metastasis of cancer[1].Previous studies have reported that the infiltration levels of various immune c...
关键词：PROGNOSIS cancer LUNG 
Resampling approaches for the quantitative analysis of spatially distributed cells
《Data Intelligence》2024年第1期104-119,共16页Giorgio Bertolazzi Michele Tumminello Gaia Morello Beatrice Belmonte Claudio Tripodo 
This study has been supported by the Italian Ministry of Education,University and Research(MIUR)through the“PON Research and Innovation 2014–2020”to G.B.;by the National Biodiversity Future Center(NBFC)CN00000033(CUP UNIPA B73C22000790001),and through the OBIND project N.086202000366(CUP G29J18000700007)to M.T.;by the Italian Foundation for Cancer Research(AIRC)through the 5×1000 I.D.22759 Grant and AIRC Accelerator Award ID.24296 to C.T.;by the Italian Ministry of Education,University and Research(MIUR)Grant 2017K7FSYB to C.T.
Image segmentation is a crucial step in various image analysis pipelines and constitutes one of the cutting-edge areas of digital pathology.The advent of quantitative analysis has enabled the evaluation of millions of...
关键词：Digital pathology spatial cytometry spatial clustering spatial randomness RESAMPLING 
Targeting T regulatory(T_(reg))cells in immunotherapy-resistant cancers
《Cancer Drug Resistance》2024年第1期1053-1077,共25页Pavlina Spiliopoulou Paramjit Kaur Tracey Hammett Giusy Di Conza Michael Lahn 
Primary or secondary(i.e.,acquired)resistance is a common occurrence in cancer patients and is often associated with high numbers of T regulatory(T_(reg))cells(CD4^(+)CD25^(+)FOXP3^(+)).The approval of ipilimumab and ...
关键词：Primary and secondary resistance T regulatory cells flow cytometry mass cytometry hyperprogression 
Aptamer-Based Cell-Surface Profiling with Single-Cell Resolution Enables Precise Cancer Characterization
《CCS Chemistry》2024年第1期196-207,共12页Liujun Xu Yawei Feng Tong Wang Shenhuan Li Kangli Xu Yue Sun Yi Luo Yishan Ye Yan Miao Yun Dong Zhenzhen Guo Qing Zhang Benshang Li He Huang Xue-Qiang Wang Liping Qiu Weihong Tan 
the National Key Research Program(grant nos.2021YFA0910101,2018YFC1602900,and 2019YFA0905800);the National Natural Science Foundation of China(NSFC;grant nos.21922404,22174039,22107027,and 21827811);the Science and Technology Project of Hunan Province(grant nos.2022JJ10005,2021RC4022,2019SK2201,2018RS3035,and 2017XK2103).
Molecular profiling of cell-surface proteins is a powerful strategy for precise cancer diagnosis.While mass cytometry(MC)enables synchronous detection of over 40 cellular parameters,its full potential in disease class...
关键词：molecular profiling cancer diagnosis mass cytometry aptamers machine learning 
C1M2:a universal algorithm for 3D instance segmentation,annotation,and quantification of irregular cells
《Science China(Life Sciences)》2023年第10期2415-2428,共14页Hao Zheng Songlin Huang Jing Zhang Ren Zhang Jialu Wang Jing Yuan Anan Li Xin Yang Zhihong Zhang 
the National Key Research and Development Program of China(2017YFA0700403,2017YFA0700402);the National Natural Science Foundation of China(62061160490);the Applied Fundamental Research of Wuhan(2020010601012167);the Fundamental Research Funds for the Central Universities(2019kfy XMBZ022);the Innovation Fund of Wuhan National Laboratory for Optoelectronics(WNLO)。
Cell instance segmentation is a fundamental task for many biological applications,especially for packed cells in three-dimensional(3D)microscope images that can fully display cellular morphology.Image processing algor...
关键词：3D instance segmentation irregular cells fluorescence images neural networks fluorescence intensity tissue cytometry 
Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver
《Liver Research》2023年第3期263-271,共9页Grayson W.Way Hongkun Lu Xuan Wang Derrick Zhao Carmen Camarena Devanand Sarkar Rebecca K.Martin Huiping Zhou 
supported by VA Merit Award 5 I01 BX005730;VA Research Career Scientist(IK6BX004477);National Institutes of Health Grant R01 DK104893,R01DK-057543 and 1R01AA030180;funding from NIH-NCI Cancer Center Support Grant P30 CA016059.
The liver plays an important role in both metabolism and immunity.Disruption of the hepatic immune microenvironment is closely associated with various liver diseases.To gain a better understanding of how different typ...
关键词：METABOLISM FLOW utilizing 
Mass cytometry uncovers a distinct peripheral immune profile and upregulated CD38 expression in patients with hidradenitis suppurativa
《Cellular & Molecular Immunology》2023年第8期972-975,共4页Peter Dimitrion Iltefat Hamzavi Congcong Yin Jugmohit Toor Kalpana Subedi Namir Khalasawi Angela Miller Richard Huggins Indra Adrianto Jesse Veenstra Gautham Vellaichamy Aakash Hans Steven Daveluy Mohammad Athar Wilson Liao Henry Lim David Ozog Li Zhou Qing-Sheng Mi 
funded by NIH/NIAMS(R01AR078688 to Q-SM and WL,R21AR079089 to Q-SM and IA);the Henry Ford Immunology Program(T71016 to Q-SM and T71017 to LZ).
Hidradenitis suppurativa(HS)is a chronic inflammatory skin disease with a worldwide prevalence of 0.3–4.0%,wherein abscesses and dermal sinus tracts form in intertriginous skin[1].HS remains poorly treated due to a l...
关键词：CD38 PATIENTS PATHOGENESIS 
Modeling cell contractility responses to acoustic tweezing cytometry
《Theoretical & Applied Mechanics Letters》2023年第2期132-142,共11页Suyan Zhang Zhenzhen Fan 
This work is supported by the National Natural Science Founda-tion of China(Grant No.11874280);the State Key Laboratory of Acoustics,Chinese Academy of Sciences(Grant No.SKLA202211).
Acoustic tweezing cytometry(ATC)is a recently developed method for cell mechanics regulation.Tar-geted microbubbles,which are attached to integrins and subsequently the actin cytoskeleton,anchor,amplify and transmit t...
关键词：Cell contractility Cytoskeleton contractile force Acoustic tweezing cytometry 2D dynamic modeling 
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