Circular dichroism spectra of different structures formed by the oligonucleotides  

作　　者：Xueguang Sun Enhua Cao Chunli Bai Yujian He Jingfen Qin 

机构地区：[1]Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China [2]Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第17期1456-1460,共5页

基　　金：ThisworkwassupportedbytheNinethFive_YearPlanKeyProjectGrantoftheChineseAcademyofSciences.

摘　　要：Under different conditions, oligonucleotides can form several different DNA structures such as duplex, triplex and quadruplex. All these structures exhibit an obvious difference in their CD spectra. The common characteristic is that they show a negative band at 240 nm, while in the range of 260-300 nm, the spectra are different from each other. Many factors such as chain direction, sugar puckering mode, orientation of the glycosyl bond, base stacking and sequence can affect their conformation and then show diversity and complexity in their spectra. By studying and comparing these spectra, more information about their conformations can be obtained to help predict some new structures. Furthermore, the spectra can also provide a base to study their potential biological functions.Under different conditions, oligonucleotides can form several different DNA structures such as duplex, triplex and quadruplex. All these structures exhibit an obvious difference in their CD spectra. The common characteristic is that they show a negative band at 240 nm, while in the range of 260–300 nm, the spectra are different from each other. Many factors such as chain direction, sugar puckering mode, orientation of the glycosyl bond, base stacking and sequence can affect their conformation and then show diversity and complexity in their spectra. By studying and comparing these spectra, more information about their conformations can be obtained to help predict some new structures. Furthermore, the spectra can also provide a base to study their potential biological functions.

关 键 词：DUPLEX TRIPLEX QUADRUPLEX CD spectrum. 

分 类 号：Q524[生物学—生物化学]