Challenge of new biological energy resources——Arguments on structure of active site of NiFe hydrogenase  

作　　者：Qi Zhang Daizheng Liao Genglin Wang 

机构地区：[1]Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第20期1673-1681,共9页

摘　　要：Hydrogenases are enzymes that can reversibly split molecular hydrogen. Study on the structure of the active site and the mechanism of catalysis has drawn great attention because the results may be useful for the design of cheap biomimetic hydrogen catalysts for fuel cells, or as model for the photoproduction of H\-2. At one time the active site was generally considered to be composed of mononuclear nickel complex with ligands from the polypeptide. A breakthrough in the understanding of the structure of Hases occurred with the resolution crystal structure of D. gigas Hases in 1995. The unexpected result challenged the previously reported spectroscopic studies and caused some academic arguments. Some methods and results used for insight into Hases have to be reconsidered. Different viewpoints concerning the structure of active site of Hases in different periods and some remaining questions will be presented.Hydrogenases are enzymes that can reversibly split molecular hydrogen. Study on the structure of the active site and the mechanism of catalysis has drawn great attention because the results may be useful for the design of cheap biomimetic hydrogen catalysts for fuel cells, or as model for the photoproduction of H2. At one time the active site was generally considered to be composed of mononuclear nickel complex with ligands from the polypeptide. A breakthrough in the understanding of the structure of [NiFe] Hases occurred with the resolution crystal structure ofD. gigas [NiFe] Hases in 1995. The unexpected result challenged the previously reported spectroscopic studies and caused some academic arguments. Some methods and results used for insight into [NiFe] Hases have to be reconsidered. Different viewpoints concerning the structure of active site of [NiFe] Hases in different periods and some remaining questions will be presented.

关 键 词：HYDROGENASES nickel_iron COMPLEXES HETERODINUCLEAR cluster active SITE fuel cells. 

分 类 号：TM911.45[电气工程—电力电子与电力传动]