Secretory expression of the fusion protein composed of human IL-2 and PreS antigen of hepatitis B virus in mammalian cells  

作　　者：Zhangguo Chen Dalong Ma Jing Wang Yingmei Zhang Yong Yuan Wenling Han Hongtao Liu 

机构地区：[1]Beijing Med Univ, Dept Immunol, Beijing 100083, Peoples R China

出　　处：《Chinese Science Bulletin》1998年第9期781-784,共4页

摘　　要：In order to make entire HBV preSAg secrete from mammalian cells, we constructed an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high hydrophilic amino acids as the linker between IL-2 C end and preSAg N end. As a result, the IL-2preS fusing protein could be secreted from mammalian cells transfected with the reconstructed vector and the expression efficiency was identical to that of natural IL-2. It was considered that the retentive effect of preS1Ag could be successfully bypassed. The results not only laid a theoretical and practical foundation for constructing specific gene vaccine against HBV persistent infection, but also supplied experimental evidence for studying modulation of protein secretory expression.In order to make entire HBV pmSAg secrete from mammalian cells, we conatmcted an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high hydmphilic amino acids as the linker between IL-2 C end and preSAg N end. As a result, the IL-2preS fusing protein could be secreted from mamalian cells transfected with the reconstructed vector and the expression efficiency was identical to that of natural IL-2. It was considered that the retentive effect of preSlAg could be successfully bypassed. The results not only laid a theoretical and practical foundation for constructing specific gene vaccine against HBV persistent infection, but also supplied experimental evidence for studying modulation of protein secretory expression.

关 键 词：hepatitis B GENE vaccine GENE IMMUNIZATION PRES antigen MAMMALIAN cells secretion. 

分 类 号：Q789[生物学—分子生物学]