Effect of TNF-α and IFN-α on the proliferation and cytotoxicity of lymphokine-activated killer in patients with bladder cancer  

作　　者：王志平 陈一戎 郑荣梁 秦大山 刘国栋 

出　　处：《Chinese Medical Journal》1997年第3期20-23,共4页中华医学杂志（英文版）

摘　　要： Objective To investigate the effects of interleukin 2 (IL 2) combined with either tumor necrosis factor α (TNF α) or α interferon (IFN α) on the proliferation and cytolysis to bladder tumor cells of lymphokine activated killer (LAK) cells in patients with bladder cancer. Methods LAK cells were generated by ficoll paque density centrifugation from 21 patients with bladder cancer and cultured in medium containing IL 2. LAK cell proliferation was assayed in the presence of various concentrations of either TNF α or IFN α by cell count in 96 well plates. Bladder cancer cell lines BIU 87 and EJ were cultured as target cells and the cytotoxicity of LAK cells was determined by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. Results The proliferation of LAK cells induced by IL 2 was enhanced by TNF α in a dose responsive fashion. The direct growth support for the LAK cells was also observed with IFN α at the concentration of 1000 U/ml after 48 hours of culture. TNF α (5000 U/ml) resulted in an increase in the cytotoxicity of LAK cells to BIU 87 and EJ cells. However, the change of cytotoxicity of LAK cells treated with IFN α was not statistically significant. Conclusions TNF α and IFN α enhance the proliferation and activation of LAK cells and influence their antitumor cytotoxicity in patients with bladder cancer.Objective To investigate the effects of interleukin 2 (IL 2) combined with either tumor necrosis factor α (TNF α) or α interferon (IFN α) on the proliferation and cytolysis to bladder tumor cells of lymphokine activated killer (LAK) cells in patients with bladder cancer. Methods LAK cells were generated by ficoll paque density centrifugation from 21 patients with bladder cancer and cultured in medium containing IL 2. LAK cell proliferation was assayed in the presence of various concentrations of either TNF α or IFN α by cell count in 96 well plates. Bladder cancer cell lines BIU 87 and EJ were cultured as target cells and the cytotoxicity of LAK cells was determined by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide (MTT) assay. Results The proliferation of LAK cells induced by IL 2 was enhanced by TNF α in a dose responsive fashion. The direct growth support for the LAK cells was also observed with IFN α at the concentration of 1000 U/ml after 48 hours of culture. TNF α (5000 U/ml) resulted in an increase in the cytotoxicity of LAK cells to BIU 87 and EJ cells. However, the change of cytotoxicity of LAK cells treated with IFN α was not statistically significant. Conclusions TNF α and IFN α enhance the proliferation and activation of LAK cells and influence their antitumor cytotoxicity in patients with bladder cancer.

分 类 号：R737.14[医药卫生—肿瘤]