Preparation of glucosamine by hydrolysis of chitosan with commercial α-amylase and glucoamylase  

作　　者：Sai-kun PAN Sheng-jun WU Jin-moon KIM 

机构地区：[1]Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China [2]School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China [3]Food Safety and Inspection Service, Office of Field Operations, US Department of Agriculture, Albany, iVY 12203, USA

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2011年第11期931-934,共4页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. 2009HS07) supported by the Open Fund of Jiangsu Key Laboratory of Marine Biotechnology,China

摘　　要：Objective： In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. Methods： Glucosamine was prepared by hydrolyzing chitosan, em- ploying e-amylase initially, and subsequently, glucoamylase. Results： The optimal hydrolyzing conditions were as follows： reaction time, 4 h; pH, 5.0; temperature, 50 ℃; and, α-amylase, 80 U/g for the initial reaction. Subsequently, glucoamylase was added in the presence of a-amylase. The optimal reaction conditions were found to be： reaction time, 8 h; pH, 4.5; temperature, 55 ℃; and, glucoamylase, 4000 U/g. The hydrolysates were subject to filtrating, concentrating to about 20% （w/w）, precipitating with five volumes of ethanol, and drying at 60 ℃ for 2 h. The content and the yield of glucosamine in the dried precipitate were 91.3% （w/w） and 86.2% （w/w）, respectively. Conclusions： The method developed in this study is a promising option in the preparation of glucosamine.Objective:In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine,an enzymatic hydrolysis method was developed.Methods:Glucosamine was prepared by hydrolyzing chitosan,employing α-amylase initially,and subsequently,glucoamylase.Results:The optimal hydrolyzing conditions were as follows:reaction time,4 h;pH,5.0;temperature,50 ℃;and,α-amylase,80 U/g for the initial reaction.Subsequently,glucoamylase was added in the presence of α-amylase.The optimal reaction conditions were found to be:reaction time,8 h;pH,4.5;temperature,55 ℃;and,glucoamylase,4 000 U/g.The hydrolysates were subject to filtrating,concentrating to about 20%(w/w),precipitating with five volumes of ethanol,and drying at 60 ℃ for 2 h.The content and the yield of glucosamine in the dried precipitate were 91.3%(w/w) and 86.2%(w/w),respectively.Conclusions:The method developed in this study is a promising option in the preparation of glucosamine.

关 键 词：GLUCOSAMINE o-Amylase GLUCOAMYLASE HYDROLYSIS 

分 类 号：Q819[生物学—生物工程]