financially supported by the National Key Research and Development Program of China(No.2020YFA0908000);CAMS Innovation fund for Medical Sciences(No.2021-I2M-1–029)。
C-Oligosaccharides are rare in nature and possess diverse bioactivities.However,their chemical synthesis faces many challenges.In this work,enzymatic introduction of C-linked sugar chains to target aglycones was succe...
The purpose of this study was to obtain an engineered Aspergillus niger strain with high glucoamylase activity by overexpressing the glucoamylase gene glaA and a-amylase gene amyA in A.niger CICC2462. Three recombi na...
supported by the System for Poultry Production Technology,Beijing Innovation Research Team of Modern Agriculture(BAIC04–2016)
Background: The effect of amylases combined with exogenous carbohydrase and protease in a newly harvested corn diet on starch digestibility, intestine health and cecal microbiota was investigated in broiler chickens.M...
supported by the 2016 CIIT-TWAS Postdoctoral Fellowship(grant number:3240293205)
Objective: To determine inhibitory activity of methanolic leaf extract of Piper umbellatum and Persea americana(P. americana)(traditionally used in Cameroon against diabetes) on β-glucosidase, β-glucosidase, maltase...
The robustness ofA. awamori and A. oryzae as enzyme producers is exploited in fungal fermentation on agricultural solid waste. High-level production of extracellular glucoamylase, protease, cellulase and xylanase has ...
A mesophilic strain of Aspergillus niger isolated from cassava effluent samples produced extracellular glucoamylase in submerged culture containing 2% (w/v) soluble or sweet potato starch. On soluble starch medium the...
Supported by the National Natural Science Foundation of China(No.81072564) and the Project of the Jilin Province Science and Technology Institute of China(No.20090945).
Two thermostable glucoamylases were produced from Aspergillus niger B-30 by submerged fermentation. The two glueoamylases GAM-1 and GAM-2 were purified by ammonium sulfate precipitation, diethylaminoethyl- cellulose f...
Supported by "948" Program of Agriculture Ministry of China(2011-Z09);Integrated Rural Energy Development Project of Agriculture Ministry of China(2130138-018);National Special Research Fund for Non-Profit Sectors of Institute of Crop Sciences,CAAS(2060302-13)
Employing RT-PCR amplification method, the mature pepfide coding sequence of glucoamylase (amyA) gene was amplified from total RNA of Talaro- myces emersonii and the xylanase (xyrut) gene from genomic DNA ofPaenib...
Project (No. 2009HS07) supported by the Open Fund of Jiangsu Key Laboratory of Marine Biotechnology,China
Objective: In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. Methods: Glucosamine was prepared by hydrolyzing chitosan, em- ployin...
by the National Basic Research Program of China(973 Program)(Grant Nos.2007CB914301 and 2007CB 914803);the Natural Science Foundation of China(Grant Nos.30940015,30770428,21002052 and 31170684);the TBR Program(No.08QTPTJC 28200,08SYSYTC00200 and 10JCYB JC14300).
Human maltase-glucoamylase(MGAM)hydrolyzes linear alpha-1,4-linked oligosaccharide substrates,playing a crucial role in the production of glucose in the human lumen and acting as an efficient drug target for type 2 di...