Alzheimer病与APOE基因的临床研究  

Clinical Study of Alzheimer's Disease and APOE Genotyping

作　　者：许家驹[1] 吴敏生[2] 伍毅 张良栋[4] 黄德仁 乔有华[6] 余彪 

机构地区：[1]上海第二医科大学精神医学教研室,200090 [2]上海杨浦区中心医院 [3]上海杨浦区精神卫生中心 [4]上海市精神卫生中心 [5]第二军医大学长海医院 [6]复旦大学遗传研究所,200433

出　　处：《中国民康医学》1997年第3期129-131,191,共4页Medical Journal of Chinese People’s Health

基　　金：上海市科技发展重点项目;复旦大学利诚基金

摘　　要：研究Alzheimer病与APOE基因相互关系，以MID作对照。取研究对象静脉血，用聚合酶链式反应（PCR）和CfoI内切酶作载脂蛋白E（APOE）基因的限制性片段长度多态性（RFLP）分析，并观察临床症状、检查神经系统体征、CT及BSSD量表评分．结果APOEε4等位基因频率AD组为18．75％（21／112），MID组为6．67％（6／90），P＜0．01，相差非常显著．提示在上海地区的AD和MID的人群中，病人的APOEε4等位基因与AD的发病可能有较为密切的关联，但未能发现APOEε4等位基因患者有特异性临床症状。With the multi-infarct dementia (MID) as the control group, the relationship between Alzheimer's disease (AD) and APOE genotyping was studied. Using the venous blood of the sub jects, with the polymerase chain reaction(PCR)and the CFOI enzym, the restriction fragment length polymorphisin (RELP) of apolipoprotein E gene was analysed, meanwhile the clinical symptoms, neurological sings, CT and rating scores of BSSD of the subjects were conducted. The results showed that there was significant difference betwetn the APOE.4 allele in AD and MID group. It suggests that in AD and MID of the people of Shanghai area, there was a close relationship of development betweenAPOE 4 allele and the AD. But the special symptoms of APOEε4 allele in the patients were not found.

关 键 词：Alzheimer病(AD) 多发脑梗塞性痴呆(MID) 载脂蛋白E(APOE)基因 

分 类 号：R749.1[医药卫生—神经病学与精神病学]