当归A_3活性部位对小鼠巨噬细胞环氧化酶-2活性及基因表达的影响  

Effect of angelica A_3 active component on activity and expression of cyclooxygenase-2 in mouse RAW264.7 macrophage cells

作　　者：沈建芬[1] 张又枝[2] 肖军花[2] 王嘉陵[2] 

机构地区：[1]浙江省嘉兴市第一医院,浙江嘉兴314000 [2]华中科技大学同济医学院基础医学院药理学系,武汉430030

出　　处：《中国药理学通报》2011年第11期1574-1577,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30171095)

摘　　要：目的研究当归A3活性部位对小鼠巨噬细胞RAW264.7环氧化酶-2(cyclooxygenase-2,COX-2)活性及基因表达的影响。方法采用酶联免疫法(enzyme-line immu-nosorbnent assay,ELISA)检测前列腺素E2(prostaglandin E2,PGE2)产量及COX-2活性,采用RT-PCR和Western blot法检测COX-2 mRNA及蛋白表达水平。结果 A3(20、40、80mg.L-1)剂量依赖性地抑制LPS诱导的RAW264.7细胞PGE2产量、COX-2活性、COX-2 mRNA及蛋白表达水平增高。结论 A3能够直接抑制PGE2产量,此作用可能与抑制COX-2基因表达有关。Aim To investigate effects of angelica A3 active component（A3） on cyclooxygenase-2（COX-2） activity and expression in lipopolysaccharide（LPS）-induced mouse RAW264.7 macrophage cells.Methods Prostaglandin E2（PGE2） production and COX-2 enzyme activity were investigated by enzyme-line Immunosorbnent assay（ELISA）.RT-PCR and Western blot were used to analyze cyclooxygenase-2 mRNA and the protein expression level.Results A3 20,40,80 mg·L-1 was shown to markedly inhibit the LPS-induced PGE2 production,COX-2 enzyme activity,COX-2 mRNA and protein expression in RAW264.7 cells（P0.05）.Conclusion A3 can directly decrease PGE2 production,which may be related to inhibiting COX-2 gene expression.

关 键 词：当归 A3活性部位 环氧化酶-2 脂多糖 前列腺素E2 巨噬细胞 

分 类 号：R-332[医药卫生] R284.1