7TD1细胞及其克隆对IL-6的反应性和依赖性  

Reactivity and Dependence of 7TD1 Cell and its Clone on IL-6

作　　者：巩新[1] 边疆[1] 薛冲[1] 陶好霞[1] 孙澎[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》1996年第4期152-155,共4页Letters in Biotechnology

摘　　要：比较了基础所和北医大提供的两株7TD1细胞。选择北医大细胞株进行克隆,筛选出对IL-6增殖反应性和依赖性较强的3株细胞(7TD1 MC1、7TD1 MC4、7TD1MC5)。其中,7TD1 MC5细胞的反应曲线呈典型的“倒S”形,对IL-6有很好的反应性和依赖性。MTT比色法显示了与~3H-TdR掺入法一致的结果,采用7TD1 MC5细胞及MTT比色法测定IL-6生物活性,可减少仪器测试的费用和避免放射性污染。确定了培养基中IL-6的最适浓度(2ng/ml)。细胞传代培养和冻存6个月,7TD1 MC5细胞对IL-6的反应性和依赖性均无明显变化。Two 7TD1 cell lines provided by Institute of Basic Medical Sciences and Beijing Medical University were compared. Cloning with the cell line from Beijing Medical University, three cell strains (7TD1 MC1, 7TD1 MC4 and 7TD1 MC5) which showed stronger growth-reactivity and dependence on IL-6 were screened. One of them, 7TD1 MC5, revealed reaction curve of typical 'reversed S', representing good reactivity and dependence on IL-6. Using 7TD1 MC5 cell and MTT method to detect biological activity of IL-6, results were correspond to those from H-TdR method while the cost was reduced and radioactive pollution was avoided. The optimal concentration of IL-6 in medium was determined to be 2ng/ml. No obvious variation of reactivity and dependence of 7TD1 MC5 cell on IL-6 was observed after 6 months Subculture and Cryostorage.

关 键 词：7TD1细胞 克隆 IL-6 反应性 依赖性 

分 类 号：Q813.2[生物学—生物工程]