Natural killer cell cytotoxicity assay with time-resolved fluorimetry  

作　　者：李建中 章竹君 金伯泉 田方 

机构地区：[1]Department of Chemistry, Nanjing University, Nanjing 210008, China [2]Department of Chemistry, Shaanxi Normal University, Xi' an 710062, China [3]Immunology Group, The Fourth Military Medical College, Xi' an 710032, China

出　　处：《Science China(Life Sciences)》1996年第2期217-224,共8页中国科学（生命科学英文版）

基　　金：Project supported by the National Natural Science Foundation of China.

摘　　要：A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has advantages of higher sensitivity, time-saving, good reproducibility and has no radioactivity problems. A satisfactory result is obtained by comparing it with 51Cr release method. It demonstrates that the new marker provides an alternative to currently used radioactive markers for the assessment of in vitro cellular cytotoxicity.A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the target cell K562 with a new synthesized fluorescence marker KLUK. The method has advantages of higher sensitivity, time-saving, good reproducibility and has no radioactivity problems. A satisfactory result is obtained by comparing it with 51Cr release method. It demonstrates that the new marker provides an alternative to currently used radioactive markers for the assessment of in vitro cellular cytotoxicity.

关 键 词：TIME-RESOLVED FLUORIMETRY NATURAL KILLER CELL FLUORESCENT marker. 

分 类 号：Q25[生物学—细胞生物学]