Twist在结直肠癌中的表达及在上皮间质转化中的作用  

Expression of Twist in colorectal cancer and its role in epithelial-mesenchymal transition

作　　者：任林飞[1] 程勇[1] 贾健锋[1] 徐维[1] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016

出　　处：《第三军医大学学报》2011年第22期2381-2385,共5页Journal of Third Military Medical University

基　　金：重庆市自然科学基金(CSTC2008BB5230)~~

摘　　要：目的观察结直肠癌中Twist基因的表达以及与结直肠癌恶性生物学行为的相关性。方法采用免疫组化S-P法及逆转录-聚合酶链反应检测10例正常结直肠粘膜、25例结直肠癌癌灶中心及癌灶边缘组织、9例淋巴结转移灶中Twist、E-cadherin、Vimentin、N-cadherin表达水平。结果 Twist的表达与结直肠癌患者的性别、年龄、肿瘤原发部位、分化程度无统计学差异(P>0.05),与有无淋巴结转移、Dukes分期、TNM分期有统计学差异(P<0.05)。4种蛋白在以上4种组织中的表达差异有统计学意义(P<0.05)。Twist、Vimentin、N-cadherin在结直肠癌灶边缘组织和淋巴结转移灶中的表达高于正常结直肠粘膜和癌灶中心中的表达(P<0.05)。E-cadherin在正常结直肠粘膜和癌灶中心中的表达显著高于结直肠癌灶边缘组织和淋巴结转移灶中的表达(P<0.05)。Twist、E-cadherin、Vimentin、N-cadherin在4种组织中阳性表达率与其相应mRNA的表达水平呈显著正相关(P<0.05)。在结直肠癌及淋巴结转移灶中,E-cadherin表达水平与Twist呈负相关(r=-0.263,P<0.05;r=-0.605,P<0.05),Vimentin的表达水平与Twist呈正相关(r=0.214,P<0.05;r=0.470,P<0.05),N-cadherin的表达水平与Twist呈正相关(r=0.381,P<0.05;r=0.581,P<0.05),而E-cadherin表达水平与Vimentin呈负相关(r=-0.562,P<0.05;r=-0.591,P<0.01),E-cadherin表达水平与N-cadherin呈负相关(r=-0.267,P<0.05;r=-0.617,P<0.01)。结论 Twist可能通过调控上皮间质转化(epithelial-mesenchymail transition,EMT)从而参与了结直肠癌的浸润、转移等过程,所以结直肠癌中Twist的表达及EMT发生可能成为独立于TNM分期以外判断结直肠肿瘤预后及浸润转移的生物学指标。Objective To investigate the expression of Twist,a member of basic helix loop helix transcription family,and its relation with the malignant features in colorectal cancer.Methods Expression of Twist,E-cadherin,Vimentin,and N-cadherin were detected in 10 normal colorectal mucosa,25 central tissues and 25 peripheral tissues of colorectal cancer,and 9 lymphatic metastases with immunohistochemistry and reverse transcription polymerase chain reaction.The results were analyzed with clinical data.Results The expression of Twist protein and mRNA in central tissues of colorectal cancer was correlated with lymph node metastasis,Dukes stage,and TNM stage（P0.05）,but not with patients＇ gender,age,and site of tumor（P0.05）.For the expression of each protein,there was difference among these four kinds of tissues（P0.05）.The expression rates of Twist,Vimentin,and N-cadherin in peripheral tissues and lymphatic metastases of colorectal cancer were significantly higher than these in normal mucosa and the central tissues（P0.05）.The expression rates of E-cadherin in normal mucosa and central tissues were significantly higher than these in peripheral tissues and lymphatic metastases tissues（P0.05）.In colorectal cancer and lymphatic metastases,a significant negative correlation was noted between the expression of Twist and E-cardherin（r=-0.263,P0.05;r=-0.605,P0.05）,while positive correlations of the expressions of Vimentin（r=0.214,P0.05;r=0.470,P0.05）and N-cadherin（r=0.381,P0.05;r=0.581,P0.05）with Twist.But E-cadherin had a negative correlation with Vimentin（r=-0.562,P0.05;r=-0.591,P0.01）,and with N-cadherin（r=-0.267,P0.05;r=-0.617,P0.01）.Conclusion Twist may take part in the invasion and metastasis of colorectal cancer by regulating epithelial-mesenchymail transition（EMT）.So Twist and EMT may be the biological indexes independent of the TNM staging system to predict the prognosis,invasion and metastasis of colorectal cancer.

关 键 词：结直肠癌 浸润转移 TWIST EMT 预后指标 

分 类 号：R394.2[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]