Rapid　Isolation　of　Large　Amount　of　Plasma　VLDL　and　LDL　by　a　Two　Step　Ultracentrifugation  

作　　者：王淳本 冯友梅 宗义强 邓耀祖 冯宗忱 

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》1995年第4期198-200,共3页华中科技大学学报（医学英德文版）

摘　　要：A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and LDL were separated and purified by a further ultracentrifugation at 265 000g for 3h.This method combines the advantages of both sequential flotation ultracentri-fugation and density gradient ultracentrifugation. It can process a large volume of plasma in a short time. The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE. This rapid.economical method is of great value in practical application.A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and LDL were separated and purified by a further ultracentrifugation at 265 000g for 3h.This method combines the advantages of both sequential flotation ultracentri-fugation and density gradient ultracentrifugation. It can process a large volume of plasma in a short time. The purity of isolated VLDL and LDL was confirmed by the lipoprotein electrophoresis on agarose gel and PAGE and by the apolipoprotein electrophoresis on SDS-PAGE. This rapid.economical method is of great value in practical application.

关 键 词：ULTRACENTRIFUGATION very low density lipoprotein (VLDL) low density lipoprotein (LDL) 

分 类 号：R446.1[医药卫生—诊断学]