ULTRACENTRIFUGATION

作品数：11被引量：6H指数：2
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相关领域：生物学更多>>
相关作者：李文奇赵芹唐希灿张广照更多>>
相关机构：华南理工大学中国科学院上海生命科学研究院清华大学更多>>
相关期刊：《Advances in Microbiology》《Science Bulletin》《Acta Pharmacologica Sinica》《Biophysics Reports》更多>>
相关基金：国家自然科学基金国家重点基础研究发展计划更多>>
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Studying large biomolecules as sedimented solutes with solid-state NMR
《Biophysics Reports》2024年第4期201-212,共12页Fan Shi Tong Zhang Juan Li Chaowei Shi Shengqi Xiang 
supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB 37040200);CAS Project for Young Scientists in Basic Research(YSBR-068).
Sedimentation solid-state NMR is a novel method for sample preparation in solid-state NMR(ssNMR)studies.It involves the sedimentation of soluble macromolecules such as large protein complexes.By utilizing ultra-high c...
关键词：Sedimentation NMR Solid-state NMR ULTRACENTRIFUGATION Magic angle spinning NUCLEOSOME 
Wastewater-based epidemiological investigation of SARS-CoV-2 in Porto Alegre,Southern Brazil被引量：1
《Science in One Health》2022年第1期67-75,共9页Bruno Aschidamini Prandi Arthur Tonietto Mangini Waldemir Santiago Neto Andre Jarenkow Lina Violet-Lozano Aline Alves Scarpellini Campos Evandro Ricardo da Costa Colares Paula Regina de Oliveira Buzzetto Camila Bernardes Azambuja Lisiane Correa de Barros Trombin Margot de Souza Raugust Rafaela Lorenzini Alberto da Silva Larre Caroline Rigotto Fabrício Souza Campos Ana Claudia Franco 
supported by CAPES(No 88887.509240/2020-00);FAPERGS(21/2551-0000069-4),ACF;FSC are PQ2 CNPq fellow.
Wastewater-based epidemiology(WBE)may be successfully used to comprehensively monitor and determine the scale and dynamics of some infections in the community.We monitored severe acute respiratory syndrome coronavirus...
关键词：SARS-CoV-2 Wastewater based epidemiology Social restrictions RT-QPCR ULTRACENTRIFUGATION 
Methods for extracellular vesicle isolation from cancer cells
《Cancer Drug Resistance》2020年第3期371-384,共14页Israa Salem Nicole M.Naranjo Amrita Singh Rachel DeRita Shiv Ram Krishn Luca S.Sirman Fabio Quaglia Alexander Duffy Nicholas Bowler Aejaz Sayeed Lucia R.Languino 
Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic ...
关键词：Extracellular vesicles differential ultracentrifugation iodixanol density gradient cell culture medium plasma tissue 
Density gradient ultracentrifugation for colloidal nanostructures separation and investigation被引量：2
《Science Bulletin》2018年第10期645-662,共18页Pengsong Li Anuj Kumar Jun Ma Yun Kuang Liang Luo Xiaoming Sun 
supported by the National Natural Science Foundation of China(NSFC);the National Key Research and Development Project of China(2016YFF0204402);the Program for Changjiang Scholars and Innovative Research Team in the University(IRT1205);the Fundamental Research Funds for the Central Universities,the Long-Term Subsidy Mechanism from the Ministry of Finance and the Ministry of Education of PRC
In this article,we review the advancement in nanoseparation and concomitant purification of nanoparticles(NPs) by using density gradient ultracentrifugation technique(DGUC) and demonstrated by taking several typical e...
关键词：Density gradient ultracentrifugation Isopycnic separation Rate zonal separation Colloidal nanostructure Nanoseparation 
Identification of a Morphogenic Intermediate of the Bacteriophage Mu Baseplate
《Advances in Microbiology》2014年第16期1155-1163,共9页Nao Tsukamoto Yuko Kanazawa Yuzuki Shimamori Keiko Yoshida Shigeki Takeda 
Bacteriophage morphogenesis is a model system for investigating sequential molecular assembly. The Mu phage is one of the most classical Myoviridae. Although it is well known as a mobile genetic element, the details o...
关键词：BACTERIOPHAGE BASEPLATE SELF-ASSEMBLY Assembly INTERMEDIATE ANALYTICAL ULTRACENTRIFUGATION 
Separation of Gold Nanorods Using Density Gradient Ultracentrifugation被引量：1
《Nano Research》2011年第8期723-728,共6页Shuai Li Zheng Chang Junfeng Liu Lu Bai Liang Luo Xiaoming Sun 
Acknowledgements This work was financially supported by the National Natural Science Foundation of China （NSFC）, Beijing Natural Science Foundation （No. 2102033）, the Programfor New Century Excellent Talents in Universities, and the 973 Program （No. 2009CB939801）.
High quality gold nanorods （NRs） with a monodisperse size and aspect ratio are essential for many applications. Here, we describe how nearly monodisperse gold NRs can be separated from polydisperse samples using den...
关键词：Au nanorods aspect ratio density gradient SEPARATION surface-enhanced Raman scattering （SERS） 
Tunable Separation of Single-Walled Carbon Nanotubes by Dual-Surfactant Density Gradient Ultracentrifugation被引量：2
《Nano Research》2011年第7期623-634,共12页Pei Zhao Erik Einarsson Georgia Lagoudas Junichiro Shiomi Shohei Chiashi Shigeo Maruyama 
Acknowledgements Part of this work was financially supported by Grant- in-Aid for Scientific Research （No. 22226006 and 19054003）, ＂Development of Nanoelectronic Device Technology＂ of New Energy and Industrial Technology Development Organization （NEDO）, and the Global Centers of Excellence （COE） Program ＂Global Center for Excellence for Mechanical Systems Innovation＂. P. Z. acknowledges a scholarship granted by the China Scholarship Council and G. L. acknowledges support from the NanoJapan program funded by the National Science Foundation.
We present a systematic study of the effects of surfactants in the separation of single-walled carbon nanotubes （SWNTs） by density gradient ultracentrifugation （DGU）. Through analysis of the buoyant densities, lay...
关键词：Single-walled carbon nanotubes density gradient ultracentrifugation sodium deoxycholate sodium dodecyl sulfate 
Optimization of crude enzyme preparation methods for analysis of glutamine synthetase activity in phytoplank-ton and field samples
《Acta Oceanologica Sinica》2009年第4期65-71,共7页WANG Yujue WANG Dazhi HONG Huasheng 
The National Natural Science Foundation of China under contract No 40331004;the Ministry of Science and Technology of China under contract No 2005DFA20430
Glutamine synthetase （GS） is an important enzyme involved in nitrogen assimilation and metabolism in marine phytoplankton. However, little work has been done in situ due to the limitation of crude enzyme preparation...
关键词：glutamine synthetase ULTRACENTRIFUGATION CENTRIFUGATION ULTRAFILTRATION PHYTOPLANKTON 
Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex
《Acta Pharmacologica Sinica》2002年第2期173-176,共4页赵芹 唐希灿 
To isolate the G4 and Gl molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS: Size exclusion chromatography and ultracentrifugation were...
关键词：ACETYLCHOLINESTERASE CHROMATOGRAPHY ULTRACENTRIFUGATION rats 
Rapid　Isolation　of　Large　Amount　of　Plasma　VLDL　and　LDL　by　a　Two　Step　Ultracentrifugation
《Journal of Huazhong University of Science and Technology(Medical Sciences)》1995年第4期198-200,共3页王淳本 冯友梅 宗义强 邓耀祖 冯宗忱 
A rapid method to obtain large amount of VLDL and LDL by ultracentrifugation is described. The mixture of VLDL and LDL was isolated and concentrated from plasma by an ultracentrifugation at 265 000 g for 2h. VLDL and ...
关键词：ULTRACENTRIFUGATION very low density lipoprotein (VLDL) low density lipoprotein (LDL) 
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