Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex  

大鼠皮层乙酰胆碱酯酶G4和G1分子亚型的分离

作　　者：赵芹[1] 唐希灿[1] 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所新药国家重点实验室,上海中国200031

出　　处：《Acta Pharmacologica Sinica》2002年第2期173-176,共4页中国药理学报（英文版）

摘　　要：To isolate the G4 and Gl molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS: Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and Gl molecular forms of AChE. RESULTS: After size exclusion chromatography, two AChE active peaks were gotten. The putative molecular weights of the two highest AChE active fractions were around Mr 239 000 and Mr 68 000 respectively, which represented G4 and Gl AChE isoforms. The sedimentation coefficients of the Mr 239 000 and Mr 68 000 fractions were 10 S and 4 S, which also assigned to G4 and Gl AChE isoforms. CONCLUSION: The G4 and Gl isoforms of AChE could be gotten by size exclusion chromatography method.目的:分离大鼠皮层乙酰胆碱酯酶G4和G1分子亚型以阐明乙酰胆碱酯酶抑制剂药物的作用。方法:使用分子排阻层析和超速离心方法分离和确证乙酰胆碱酯酶的G4和G1分子亚型.结果:经过分子排阻层析得到两个乙酰胆碱酯酶的活性峰,与标准蛋白质的洗脱峰相比,推测其分子量为M_r 239000和M_r 68000.通过超速离心法确证其沉降系数为10S和4S,分别为乙酰胆碱酯酸G4和G1分子亚型.结论:乙酰胆碱酯酶的G4和G1分子亚型可以使用分子排阻层析的方法分离得到.

关 键 词：ACETYLCHOLINESTERASE CHROMATOGRAPHY ULTRACENTRIFUGATION rats 

分 类 号：R96[医药卫生—药理学]