Studying large biomolecules as sedimented solutes with solid-state NMR  

作　　者：Fan Shi Tong Zhang Juan Li Chaowei Shi Shengqi Xiang 

机构地区：[1]MOE Key Lab for Cellular Dynamics,School of Life Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230026,China [2]Hefei National Laboratory for Physical Sciences at Microscale,University of Science and Technology of China,Hefei 230026,China

出　　处：《Biophysics Reports》2024年第4期201-212,共12页生物物理学报（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB 37040200);CAS Project for Young Scientists in Basic Research(YSBR-068).

摘　　要：Sedimentation solid-state NMR is a novel method for sample preparation in solid-state NMR(ssNMR)studies.It involves the sedimentation of soluble macromolecules such as large protein complexes.By utilizing ultra-high centrifugal forces,the molecules in solution are driven into a high-concentrated hydrogel,resulting in a sample suitable for solid-state NMR.This technique has the advantage of avoiding the need for chemical treatment,thus minimizing the loss of sample biological activity.Sediment ssNMR has been successfully applied to a variety of non-crystalline protein solids,significantly expanding the scope of solid-state NMR research.In theory,using this method,any biological macromolecule in solution can be transferred into a sedimented solute appropriate for solid-state NMR analysis.However,specialized equipment and careful handling are essential for effectively collecting and loading the sedimented solids to solid-state NMR rotors.To improve efficiency,we have designed a series of loading tools to achieve the loading process from the solution to the rotor in one step.In this paper,we illustrate the sample preparation process of sediment NMR using the H1.4-NCP^(167) complex,which consists of linker histone H1.4 and nucleosome core particle,as an example.

关 键 词：Sedimentation NMR Solid-state NMR ULTRACENTRIFUGATION Magic angle spinning NUCLEOSOME 

分 类 号：Q6-3[生物学—生物物理学]