EFFECT OF rhTGF-β1 AND rhGM-CSF ON RECEPTOR EXPRESSIONS IN J6-1 AND J6-2 LEUKEMIC CELLS  

作　　者：郑国光[1] 

机构地区：[1]中国医学科学院血液学研究所,天津300020

出　　处：《中国实验血液学杂志》1995年第3期274-279,共6页Journal of Experimental Hematology

基　　金：Supported by National Natural Sciences Foundation. The abstract of this work was published in Exp Hematol (1994:22:743)

摘　　要：ABC immunoperoxidase was used to test the effects of rhTGF-β1 and rhGM-CSF on receptor expressions in J6-1 and J6-2 leukemic cell lines. Computer assisted image analysis system was introduced to evaluate positive index of time-and dose-dependent specimens. The expression of c-kit was elevated both in positive rate and positive index by TGF-01 in both time- and dose-dependent manners. Ing/ml rhTGF-β1 simultaneously enhanced the expression of c-fms and PDGF-R which is not detected in 50 ng / ml GM-CSF treatment. Endoglin was down-regulated after TGF-β treatment and up-regulated in J6-2 cells after GM-CSF treatment, c-kit Expression was elevated by TGF-β in J6-1 cells while decreased by both in J6-2 cells.ABC immunoperoxidase was used to test the effects of rhTGF-β1 and rhGM-CSF on receptor expressions in J6-1 and J6-2 leukemic cell lines. Computer assisted image analysis system was introduced to evaluate positive index of time-and dose-dependent specimens. The expression of c-kit was elevated both in positive rate and positive index by TGF-01 in both time- and dose-dependent manners. Ing/ml rhTGF-β1 simultaneously enhanced the expression of c-fms and PDGF-R which is not detected in 50 ng / ml GM-CSF treatment. Endoglin was down-regulated after TGF-β treatment and up-regulated in J6-2 cells after GM-CSF treatment, c-kit Expression was elevated by TGF-β in J6-1 cells while decreased by both in J6-2 cells.

关 键 词：TGF-Β GM-CSF RECEPTOR expression leukemic cell IMAGE analysis 

分 类 号：R33[医药卫生—人体生理学]