用原位聚合酶链反应检测肝癌石蜡包埋组织中的HBV—DNA  

DETECTION OF HBV DNA IN LIVER CANCER TISSUES WITH IN SITU POLY-MERASE CHAIN REACTION

作　　者：蒋明德[1] 褚桂珍[1] 曾维政[1] 张建华[1] 陈泰庆 楚人俊 张家碧 

机构地区：[1]成都军区总医院消化内科,成都610083

出　　处：《西南国防医药》1995年第2期65-67,2,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：为研究乙型肝炎病毒(HBV)感染和肝细胞癌(HCC)之间的关系,我们建立了原位聚合酶链反应(ISPCR)并检测26例HCC石蜡包埋组织中的HBV—DNA。结果显示,HBV DNA在肝癌组织和癌旁肝组织的阳性率分别为84.6％(22/26)、91.3％(21/23)。ISPCR技术既显示了PCR技术的高敏性和特异性,又能在细胞内定位,是研究HBV与HCC关系的一项有价值的技术。In order to study the relationship between hepatitis B virus ( HBV) infection and hep-atocellular carcinpma (HCC) . HBV DNA in formalin -fixed paraf if in-embedded liver tissues of 26 patients with HCC had been detected with in situ polymerase chain reaction ( ISPCR). The results showed that the positive rate of HBV DNA in the intrahepatic tissues was 84. 6% (22/26)and in the peritumor tissues was 91. 3% (21/23). So it is suggested that ISPCR is of sensitivity and specificity as well as precise localization to HBV DNA and it is valuable in investigating the relationship between HBV and HCC.

关 键 词：原位聚合酶链反应 肝细胞瘤 HBV—DNA 

分 类 号：R735.7[医药卫生—肿瘤]