幽门螺杆菌培养滤波诱导胃粘膜细胞DNA损伤  

DNA DAMAGE OF GASTRIC MUCOSAL CELLS INDUCED BYCONCENTRATED HELICOBACTER PYLORI CULTURE SUPERNATANTS

作　　者：梁后杰[1] 刘为纹[1] 房殿春[1] 王俊茹[1] 周子成[1] 王振华[1] 时超美 徐启旺[1] 

机构地区：[1]重庆市第三军医大学西南医院,重庆市第三军医大学临床微生物教研室

出　　处：《中华消化杂志》1995年第S1期21-22,共2页Chinese Journal of Digestion

摘　　要：本试验以布氏肉汤旋转培养法制备幽门螺杆菌（ＨＰ）培养滤液，研究Ｈｐ代谢物体外对胃粘膜细胞ＤＮＡ的损伤作用。结果表明，胃粘膜细胞受Ｈｐ培养滤液作用后，双链ＤＮＡ百分含量，ＤＮＡ增色效应和ＤＮＡ－溴化乙锭复合物的荧光强度随作用时间增加而降低，受损ＤＮＡ对脱氧核糖核酸酶产生明显抗性，提示Ｈｐ的代谢物可引起胃粘膜细胞ＤＮＡ损伤，Ｈｐ感染可能是胃癌的重要病因之一。This study was to investigate the DNA damage of gastric mucosal cells induced by concentrated H.pylori culure supernatants.Results showed that concentrated H.pylori culture supernatants could cause DNA damage of gastric mucosal cells,as indicated by the decrease of double-strand DNA percentage,hyperchromaticity,fluorescence intensity of DNA-EB complex with EB as the fluorescent probe and resistance to hydrolysis by DNase I.These findings suggest that some substances secreted by H.pylori could cause DNA damage and H.pylori infection may be one of the etiologic factors of gastric cancer.

关 键 词：幽门螺杆菌 胃粘膜细胞 脱氧核糖核酸损伤 

分 类 号：R363[医药卫生—病理学]